Team:Leeds/Parts

From 2013.igem.org

Revision as of 03:47, 5 October 2013 by Parsley (Talk | contribs)

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Back to iGEM Main Page
Leeds' Registry Parts
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Please check back here frequently for a full list of the parts we've developed, submitted to the [http://partsregistry.org Registry] and have had approved. We will also endeavour to include our characeterisation protocols, although we are currently collaborating with Purdue to decide exactly what these should be.


Contents

Currently Working On

  • [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1028000 Silica Binding Peptide (Si4):] A short amino acid sequence that binds silica with high affinity.
  • [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1028002 Bio-system 1:] CpxR promoter controlling GFP expression. Will produce the green fluorescent protein following exposure to hydrophobic membrane stress. Extensive experimental data has been acquired about the response of the cpxR promoter to various forms of physical and chemical stresses, this can be found in the biosystem 1 section of the Lab Results page.
  • [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1028003 Bio-system 2:] INP.Si4 is produced constitutively with GFP production under control of CpxR promoter. The system is designed to bind with high affinity to silica macro-structures and produce a fluorescent response when it does. Experimental data has been gathered for this part in unison with the silica binding peptide. Problems with expression meant that the data is not as conclusive as predicted but indicates that Si4 does bind silica with a degree of affinity.


Submitted

A full listing of all parts Leeds iGEM 2013 have submitted to the Registry, and their status.
<groupparts>iGEM013 Leeds</groupparts>

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Geneious, our fine sponsors and suppliers of software Bioline, our fine sponsors and suppliers of equipment Qiagen, our fine sponsors and suppliers of PCR kits
Bangs Laboratories, our fine sponsors and suppliers of silica beads
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