Team:TU-Munich/Results/Overview

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Results Overview

Having spent our summer in the lab, we are proud to present our accomplishments. Previous to the Euopean regional jamboree in Lyon, we have created 72 BioBricks and devices, transformed and selected 20 different transgenic GM-mosses and characterized our effectors as recombinant proteins produced in E. coli and in our moss Physcomitrella patens, a chassis newly introduced into iGEM. We took further steps to put our phytoremediation project into practise by developing concepts for the implementation of our PhyscoFilter in the environment and by analyzing the economic potential of this innovative technology in our Entrepreneurship section. Additional to our wetlab work, we contributed a very powerful software tool for the annotation of BioBricks from the parts registry and contributed tutorials to pass this summer´s experiences and skills on to the subsequent iGEM generations.

BioBricks

We created 72 BioBricks, including BioBricks advancing the use of Physcomitrella patens as a chassis, BioBricks enhancing phytoremediation applications and BioBricks for the light triggered kill-switch mechanism. (Read more)

Effectors

We selected six different effector proteins and produced them in Escherichia coli for their further characterization concerning stability and activity. (Read more)

WT-Moss

We performed some general experiments to optimize Physcomitrella patens concerning tolerance of toxins, growth optimization and the use of different cultivation surfaces. (Read more)

GM-Moss

We created 20 different strains of transformated moss during our visit to Prof. Reski´s lab in Freiburg. After selection and regeneration transgenic, we started the experiments. (Read more)

Software

Protein coding BioBricks constitute large parts of the Parts Registry. We created the AutoAnnotator for convenient in-silico translation of their sequences and provision of various valuable parameters summed up in a formatted table. (Read more)

RFC 96

To improve the standardization of protein-encoding Biobricks, we introduce the AutoAnnotator, a software tool that calculates several information by using the DNA sequence of the Biobricks as a blueprint to display them in a standard table.
Read more

Tutorials

During the summer we learned a lot about iGEM and found solutions for problems which appear in many iGEM projects. So we want to share this knowledge for the benefit of the community. Therefore we created our tutorial section.
Read more

Entrepreneurship

Science is an extremely important point for progress, but in the end it is essential to develop a business model oppon which the technology can be brought to market.
Read more

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