Team:USTC CHINA/Notebook/Protocols

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Gene Clone

Gel Extraction

DNA digestion

Single Digestion

Double Digestion

Colony PCR

PCR

Ligation

Plasmid mini-prep

Transformation

The transformation of E.coli

The transformation of Bacillus subtilis

Overlap PCR

Extracting plasmids

Extracting plasmids from gram-positive bacterium

Extracting plasmids from gram-negative bacterium

Expression of proteins

Expression of proteins in Bacillus subtilis

Expression of proteins in Escherichia coli

Concentrating proteins

Transdermal experiments Immunity&Tests

ELISA

Sample analysis

Medium

LB media

10*S base

HS media

LS media

2*YT media

Measurement

Constitutive promoter measurements

Plac promoter response to IPTG or Glucose

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