Team:Leeds/Results

Bacterial growth curves

We are monitoring the growth of our bacterial devices to see if any of the genes we have inserted have an effect on bacterial growth.

Control Bacterial Growth Curve

This growth curve shows the lag phase and log phase of untransformed bacteria. The graph also shows that the bacteria do not grow in the presence of chloramphenecol. Our bacterial device will grow in the presence of chloramphenecol. We will use this graph as a comparison with our bacterial device.

Agarose Gels For Digestions

For every Digestion we do we will run a gel to ensure the digestion was successful, and use gel extraction kits to obtain purified DNA.

Digestion of plasmid containing Green Flourescent Protein

The part [http://parts.igem.org/Part:BBa_K081012:Design K081012] was digested with EcoR1 and a gel run to make sure the fragment(s)are of the correct length.

DNA Ladder Calibration Graph

From this graph we can work out that one fragment is around 2800bp long, while the other appears to be 4000bp long - far longer than the plasmid at 2858bp long. Through digestion, it has been linearised by a single EcoR1 digest. The fragment corresponds to what we expect so our digest was succesful, despite not all of the plasmid being digested.

Characterisation of Device 1

Growth Curve

Restriction Digests

File:Leeds BS1Restrictiondigests.png

Plate Imaging

Membrane Stress - Hydrophobic Stress

Membrane Stress - Changes in pH

Membrane Stress- Atomic Force Microscopy

Characterisation of Device 2

Growth Curve

Restriction Digests

Plate Imaging

Binding assay-washing beads

Binding assay-Confocal Microscopy

Characterisation of Device 3

Growth Curve

Restriction Digests

Plate Imaging

Binding Assay-Beads cause fluorescence?