From 2013.igem.org

• Project: Plasticity
  • At a glance
  • Waste Issue
  • M.A.P.L.E. Modules
    • M1: Resource-full Waste
    • M2: Plastic Fantastic
  • Bioplastic applications
• Human Practices
  • M.A.P.L.E
  • Industrial Implementation
  • Social Challenge
  • Communicating our Project
  • Project Evolution
• Achievements
  • Judging Criteria
  • Main Results
  • Collaborations
  • Biobricks
• In the Lab
  • Safety
  • Lab book
  • Modelling Notes
    • Introduction
    • Beta amyloid degradation
  • Data
    • Growth Assays
    • Western Blot
    • Enzyme Kinetics
    • PHB production
    • 3HB assay
    • Clearing Assays
    • Electron microscopy
  • Protocols
• Team
  • Students
  • Advisors
  • Sponsors
  • Attributions

Results

Glucose growth assay

Cell growth of phaABC E. coli at 4 concentrations of glucose. Optimum growth is at 2-4% glucose at 37ºC.

WCM growth assay

Production of the red pigment by stress induction. MG1655 were grown with LB media and sterile filtrated WCM

Growth curve of our [http://parts.igem.org/Part:BBa_K639003 mCherry] MG1655 bacteria. MG1655 were grown with LB media and sterile filtrated WCM at 37ºC

Ethylene glycol growth assay

Cell growth of MG1655 in ethylene glycol, a byproduct of polyurethane degradation. Cells were grown in 0µl, 100µl and 200µl at 30ºC.

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