From 2013.igem.org

• Project: Plasticity
  • At a glance
  • Waste Issue
  • M.A.P.L.E. Modules
    • M1: Resource-full Waste
    • M2: Plastic Fantastic
  • Bioplastic applications
• Human Practices
  • M.A.P.L.E
  • Industrial Implementation
  • Social Challenge
  • Communicating our Project
  • Project Evolution
• Achievements
  • Judging Criteria
  • Main Results
  • Collaborations
  • Biobricks
• In the Lab
  • Safety
  • Lab book
  • Modelling Notes
    • Introduction
    • Beta amyloid degradation
  • Data
    • Growth Assays
    • Western Blot
    • Enzyme Kinetics
    • PHB production
    • 3HB assay
    • Clearing Assays
    • Electron microscopy
  • Protocols
• Team
  • Students
  • Advisors
  • Sponsors
  • Attributions

Results

Glucose growth assay

Cell growth of phaABC E. coli at 4 concentrations of glucose. Optimum growth is at 2-4% glucose at 37ºC.

WCM growth assay

Production of the red pigment by stress induction. MG1655 were grown with LB media and sterile filtrated WCM for 48 hours.

Growth curve of our [http://parts.igem.org/Part:BBa_K639003 mCherry] MG1655 bacteria. MG1655 were grown with LB media and sterile filtrated WCM at 37ºC

Ethylene glycol growth assay

Cell growth of MG1655 in ethylene glycol, a byproduct of polyurethane degradation. Cells were grown in 0mM, 100mM or 200mM Ethylene Glycol at 30ºC.

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