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User contributions
From 2013.igem.org
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- 11:14, 16 September 2013 (diff | hist) Team:Leicester/Notebook
- 10:23, 30 August 2013 (diff | hist) 30/08/13 (→Repeating the combustion experiment)
- 10:22, 30 August 2013 (diff | hist) N 30/08/13 (Created page with "==Preparing samples to be sent to be sequenced by PNACL== ==Repeating the combustion experiment== *Blocks of both DNA and no DNA coating were made *Block thicker than last experi...")
- 10:20, 30 August 2013 (diff | hist) Team:Leicester/Notebook
- 10:19, 30 August 2013 (diff | hist) 29/08/13
- 14:02, 29 August 2013 (diff | hist) 31/07/13 (→Results from the Transformation of limonene Biobrick (BBa_K118025) to the pSB1C3 vector) (top)
- 14:01, 29 August 2013 (diff | hist) 28/08/13 (→DNA extraction from gel) (top)
- 14:01, 29 August 2013 (diff | hist) 28/08/13 (→DNA extraction from gel)
- 14:00, 29 August 2013 (diff | hist) 29/08/13 (→Run Tod operon ligation genes on the gel)
- 13:59, 29 August 2013 (diff | hist) N 29/08/13 (Created page with "==Make 2% agarose gel== ==Run Tod operon ligation genes on the gel== ==Isolate the DNA from gel== *DNA isolated using Bioline Isolate II PCR and gel kit and following its protocol")
- 13:57, 29 August 2013 (diff | hist) Team:Leicester/Notebook
- 13:55, 29 August 2013 (diff | hist) 28/08/13 (→gel electrophoresis)
- 11:43, 28 August 2013 (diff | hist) Team:Leicester/Notebook
- 11:35, 23 August 2013 (diff | hist) N File:Igem ovenight incub herrinngDNA 230813.tif (top)
- 11:34, 23 August 2013 (diff | hist) 23/08/13 (→Running overnight dilutions on gel)
- 11:34, 23 August 2013 (diff | hist) 23/08/13
- 11:15, 23 August 2013 (diff | hist) 23/08/13 (→Nanodropping overnight incubations of herring sperm DNA)
- 11:14, 23 August 2013 (diff | hist) 23/08/13 (→Nanodropping overnight incubations of herring sperm)
- 11:14, 23 August 2013 (diff | hist) 23/08/13 (→Nanodropping overnight incubations of herring sperm)
- 11:14, 23 August 2013 (diff | hist) 23/08/13 (→Nanodropping overnight incubations of herring sperm)
- 11:10, 23 August 2013 (diff | hist) 23/08/13 (→Nanodropping overnight incubations of herring sperm)
- 11:10, 23 August 2013 (diff | hist) 23/08/13 (→Nanodropping overnight incubations of herring sperm)
- 11:09, 23 August 2013 (diff | hist) 23/08/13 (→Nanodropping overnight incubations of herring sperm)
- 11:07, 23 August 2013 (diff | hist) 23/08/13 (→Nanodropping overnight incubations of herring sperm)
- 11:06, 23 August 2013 (diff | hist) 23/08/13 (→Nanodropping overnight incubations of herring sperm)
- 11:06, 23 August 2013 (diff | hist) 23/08/13 (→Nanodropping overnight incubations of herring sperm)
- 11:05, 23 August 2013 (diff | hist) N 23/08/13 (Created page with "==Nanodropping overnight incubations of herring sperm== *Table representing the concentrations of herring sperm after and before different treatments {|border=1 |Sample||Concentr...")
- 09:19, 23 August 2013 (diff | hist) Team:Leicester/Notebook
- 09:07, 23 August 2013 (diff | hist) 22/08/13 (→Running sonicated herring sperm DNA on 0.8% gel)
- 09:03, 23 August 2013 (diff | hist) N File:Igem shear son 220813.jpg (top)
- 09:03, 23 August 2013 (diff | hist) 22/08/13 (→Running sonicated herring sperm DNA on 0.8% gel)
- 12:42, 21 August 2013 (diff | hist) Team:Leicester/Notebook
- 12:42, 21 August 2013 (diff | hist) 20/08/13 (→Plating out bacteria)
- 12:41, 21 August 2013 (diff | hist) 20/08/13 (→Plating out bacteria)
- 12:41, 21 August 2013 (diff | hist) N 20/08/13 (Created page with "==Making nutrient agar plates and broth== *Making up broth and agar solution to grow ''P. putida'' in. *For agar plates: **9.2g of nutrient agar medium **400ml of distilled water...")
- 14:16, 19 August 2013 (diff | hist) 19/08/13 (→Running both denatured and sonicated samples on gel)
- 14:02, 19 August 2013 (diff | hist) 19/08/13 (→Running both denatured and sonicated samples on gel)
- 14:02, 19 August 2013 (diff | hist) 19/08/13 (→Running both denatured and sonicated samples on gel)
- 13:58, 19 August 2013 (diff | hist) N File:Igem denatured son 190813.jpg (top)
- 13:57, 19 August 2013 (diff | hist) 19/08/13 (→Running both denatured and sonicated samples on gel)
- 11:04, 19 August 2013 (diff | hist) 19/08/13 (→Running both denatured and sonicated samples on gel)
- 11:04, 19 August 2013 (diff | hist) N 19/08/13 (Created page with "==Denaturing sonicated DNA== *Denaturing DNA by heat shock for 10mins in a 95C heat block *Adding 5ul of OG dye ==Running both denatured and sonicated samples on gel== *For just...")
- 11:01, 19 August 2013 (diff | hist) Team:Leicester/Notebook
- 13:40, 16 August 2013 (diff | hist) 16/08/13 (→Running the gel from sonication series)
- 13:39, 16 August 2013 (diff | hist) N File:Igem 2nd son series 160813.jpg (top)
- 13:39, 16 August 2013 (diff | hist) N 16/08/13 (Created page with "==Running the gel from sonication series== *Samples were taken from sonicated tube every 30min intervals, a total of 6 samples. *Each was diluted 100 fold to get a concentration ...")
- 13:34, 16 August 2013 (diff | hist) Team:Leicester/Notebook
- 09:20, 16 August 2013 (diff | hist) 18/07/13 (Replaced content with "==Double digest==")
- 12:01, 12 August 2013 (diff | hist) 12/08/13 (→Running an agarose gel for sonication analysis)
- 11:50, 12 August 2013 (diff | hist) 12/08/13 (→Running an agarose gel for sonication analysis)
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