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From 2013.igem.org
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- 18:42, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/4 September 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Results of the gradient PCR of the Sensing constructs from the previous day''' <BR> The Gradient PCR of the Backbones for constructs...")
- 18:40, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/3 September 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Gradient PCR of sensning constructs''' <BR> We used the new primers to run a PCR on the backbones and insert of the sensning constru...")
- 18:39, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/1 September 2013
- 18:36, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/1 September 2013
- 18:35, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/1 September 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Suspending Primers''' <BR> Our new primers arrived and we resuspended them and made primer mixes for each pair, one foreward and one...")
- 18:25, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/30 August 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Isolation of genomic MG1655 DNA''' <BR> We followed the manufactures instruction from the DNa isolation kit we recieved in order to ...")
- 18:24, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/29 August 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Results of GFP expression''' <BR> The inoculated bacteria did not grow at all in the arabinose containing medium. We thus innoculate...")
- 18:22, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/28 August 2013
- 18:21, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/28 August 2013
- 18:19, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/28 August 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Checking GFP expression induced by arabinose''' <BR> We wanted to induce simple GFP expression in bacteria transformed with the AraC...")
- 18:17, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/27 August 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Primer design''' <BR> We desinged new primers for the three sensing construct as well as for the three effector constructs. This tim...")
- 18:14, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/26 August 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''PCR of the BBa_J23119 part from iGEM''' <BR> In order to have a positive control for our sensing constructs we wanted to use a const...")
- 18:11, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/25 August 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''PCR of Cad Backbone (1.2-B)''' <BR> We tried to amplify the Backbone for the Cad-GFP construct. But it did not work. Since the restr...")
- 18:08, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/23 August 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''PCR of the effector Backbones''' <BR> Again we tried to amplifiy the backbones of the constructs containing the AraC promoter and th...")
- 18:04, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/22 August 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Gradient PCR of Sensing Backbones''' <BR> We did another gradient PCR for the backbones but for the constructs three sensing constru...")
- 18:02, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/21 August 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Transformation of Bacteria''' <BR> We did another transformation: <BR> 1.) Two plates with the AraC promoter containing Plasmid, dir...")
- 18:00, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/20 August 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Gradient PCR of Backbones''' <BR> To check which annealing temperature is ideal for our backbone amplification we did a gradient PCR...")
- 17:59, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/19 August 2013
- 17:58, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/19 August 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Gel electrophoresis of the backbones with and without GFP''' <BR> After running the gel for 1.5h, there were two visible bands for t...")
- 17:56, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/18 August 2013
- 17:54, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/18 August 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''PCR of MMP2 and MMP9 inserts and Backbones''' <BR> We did the same PCR as the previous day. It again did not work neither for the in...")
- 17:43, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/17 August 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Gel Electrophoresis''' <BR> The PCR products from the day befor were run on a 0.8% agar gel.(see Results) The MMP inserts were not ...")
- 17:42, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/16 August 2013
- 17:41, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/16 August 2013
- 17:41, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/16 August 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Making a glycerol stock''' <BR> From each culture that was inoculated we made a 50% glycerol stock of 500ul Bacterial culture and 50...")
- 17:34, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/15 August 2013
- 17:33, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/15 August 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Primer Resuspension''' <BR> We recieved our primers and resuspended them. Then we dilluted them in order to have a final concentrati...")
- 17:31, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/14 August 2013
- 17:30, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/14 August 2013
- 17:27, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/14 August 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Amplifying Plasmids containing gelatinases''' <BR> We transformed two cultures each with a plasmid containing a gene that encoded ei...")
- 17:24, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/12 August 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Miniprep of the inoculated bacteria''' <BR> We isolated the Plasmids from the bacteria by a Miniprep. {{Template:EPFL2013Footer}}")
- 17:23, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/10 August 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Making a glycerol Stick''' <BR> We made a 50% glycerol stock of the Bacteria that we innoculated the day before. {{Template:EPFL2...")
- 17:20, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/9 August 2013
- 17:20, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/8 August 2013
- 17:20, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/8 August 2013
- 17:19, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/9 August 2013
- 17:19, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/9 August 2013 (Created page with "{Template:EPFL2013Header}} =Sensing= <BR> '''Innoculating Bacteris''' We innoculated the bacteria transformed the day before in order to do a Miniprep according to the manufact...")
- 17:15, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/8 August 2013
- 17:13, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/8 August 2013 (→Sensing)
- 17:13, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/8 August 2013 (→Sensing)
- 17:12, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/8 August 2013 (→Amplifying Plasmids)
- 17:12, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/8 August 2013 (→Sensing)
- 17:12, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/8 August 2013 (→Sensing)
- 17:08, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/8 August 2013 (Created page with "==='''Sensing'''=== =Amplifying Plasmids= Trasformed DH5 alpha cells with the Plasmids containing parts BBa_I746908 (Arabinose promoter) & BBa_J23119 (pH promoter). These two pa...")
- 16:13, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/15 June 2013 (Created page with "==Nanoparticle== ==coupling== ==sensor-effector==") (top)
- 13:48, 29 September 2013 (diff | hist) Team:EPF Lausanne/Sensing-Effector
- 13:47, 29 September 2013 (diff | hist) Team:EPF Lausanne/Sensing-Effector
- 13:47, 29 September 2013 (diff | hist) N File:Team-EPF-Lausanne 1.3 Construct Map.jpg (top)
- 13:45, 29 September 2013 (diff | hist) Team:EPF Lausanne/Sensing-Effector
- 13:44, 29 September 2013 (diff | hist) Team:EPF Lausanne/Sensing-Effector
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