Team:TU-Eindhoven/Project

eColiFigure 

eColiCESTFigure 

 Abstract
 Our project presents an alternative solution to the use of heavy metals MRI contrast agents by focusing on CEST MRI. Within CEST  imaging, proteins enclosing hydrogen atoms generate high quality images.  We use Escherichia coli to create CEST proteins when the  bacteria sense a hypoxic environment due to a promoter designed for this purpose, thus working as a production and delivery system for the CEST MRI contrast agent. Hypoxic regions are related to tumors,therefore our eventual goal is to use this device to target and image tumors in humans by injecting the bacteria into the bloodstream. A second application is tracking bacteria in bacterial  infections studies. For the iGEM competition however, the proteins are only expressed ex-vivo:in aerobic and anaerobic conditions. We aim to achieve an efficient testing of the CEST properties of the proteins and confirm the promoter’s ability to express each   protein.