Team:BYU Provo/Notebook/Cholera - Enzyme/September/Period1/Dailylog

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Cholera - Enzymes Notebook: September 1 - September 14 Daily Log



Overview
March-April
May-June
July-August
September

9/4/13

Today we set up phusion and Taq PCR for B. subtilis and phusion PCR for dspB

Phusion PCR setup 

   35 microliters ddH2O
   10 microliters 5X phusion buffer
   1.5 microliters 10mM dNTP's
   1 microliter each primer
   1 microliter diluted template DNA
   .5 microliter phusion polymerase

Taq PCR setup 

   40 microliters ddH2O
   5 microliters 10X TAQ buffer
   1.5 microliters 10mM dNTP's
   1 microliter each primer
   1 microliter diluted template DNA
   .5 microliter phusion polymerase

-NRS


9/5/2013

I ran the PCR products from 9/4 on gel and there were no bands. -NRS


9/6/2013

Dezi helped us set up the PCR again using the primers to clone into the iGEM plasmid to see if our other primers are the problem. -MJS/NRS

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