Team:Evry/Protocols

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Iron coli project

Protocols Overview

  1. Competent cells
  2. Transformation
  3. Plasmid purification
  4. Solutions & Media
  5. Golden Gate Assembly
  6. Tecan Analysis
  7. PCR and electrophoresis
  8. Genomic DNA extraction
  9. Siderophore Detection
  10. Homologous Recombination
  11. PCR Purification
  12. Primer design
  13. Biobricking
  14. Repiquage

Finies ou quasi:

  • Competent cells
  • Transfo
  • Plasmid purif
  • Tecan
  • Genomic extraction
  • ChromAzurol S
  • Biobricking
  • Delta Fur
  • PCR purif
En cours:
  • Solution and Media
  • Golden Gate
  • PCR
A faire
  • Primer design
  • Repiquage
  • Microfluidique
  • Expérience pH (Gabi)
  • RT qPCR ?
  • Compétition bactéries ?
    http://barricklab.org/twiki/bin/view/Lab/ProceduresLongTermCompetitions

Faudra remettre les protocoles dans un ordre chronologique

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