Team:Paris Saclay/Notebook/August/6

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Notebook : August 6

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining Bba_K1155004, Bba_K1155005, Bba_K1155006

1 - Electrophoresis to check the Colony PCR products : Bba_K1155004, Bba_K1155005, Bba_K1155006

XiaoJing, Damir, Anaïs

  • Bba_K1155004 :
[[]]
  • Well 1 : 6µL DNA Ladder
  • Well 2 to 7 : 10µL Bba_K1155004+2µl of 6X loading dye
  • Well 8 : 6µL DNA Ladder
  • Well 9 to 14 : 10µL Bba_K1155004+2µl of 6X loading dye
  • Well 15 : 6µL DNA Ladder
  • Well 16 : 6µL DNA Ladder
  • Well 17 to 22 : 10µL Bba_K1155004+2µl of 6X loading dye
  • Well 23 : 6µL DNA Ladder
  • Well 24 to 30 : 10µL Bba_K1155004+2µl of 6X loading dye
  • Well 31 : 6µL DNA Ladder
  • Gel : 1%
  • Bba_K1155005 :
[[]]
  • Well 1 : 6µL DNA Ladder
  • Well 2 to 7 : 10µL Bba_K1155005+2µl of 6X loading dye
  • Well 8 : 6µL DNA Ladder
  • Well 9 to 14 : 10µL Bba_K1155005+2µl of 6X loading dye
  • Well 15 : 6µL DNA Ladder
  • Well 16 : 6µL DNA Ladder
  • Well 17 to 22 : 10µL Bba_K1155005+2µl of 6X loading dye
  • Well 23 : 6µL DNA Ladder
  • Well 24 to 30 : 10µL Bba_K1155005+2µl of 6X loading dye
  • Well 31 : 6µL DNA Ladder
  • Gel : 1%
  • Bba_K1155006 :
[[]]
  • Well 1 to 12 : 10µL Bba_K1155006+2µl of 6X loading dye
  • Well 13 : 6µL DNA Ladder
  • Well 14 to 26 : 10µL Bba_K1155006+2µl of 6X loading dye
  • Gel : 1%

Expected size :

  • NarK, NarG, NirB : 500 bp

We obtain fragments at the good size for all the colony. ENSUITE ?????????

2 - Samples culture

Xiaoing, Anaïs

We put in culture the 6, 7, 8 samples for every promoter in 5 ml LB + 5 µl Chlorenphenicol (1000x,20µg/ml) Incubator over night to 310,15K at 180 RPM

B - PCB sensing system

Obtaining the BphA1 promoter

1 - Sequence analysis for BB pBphA1 in psB1C3 5 ( clone 4, 17 and 22 )

IMAGE

A - Aerobic/Anaerobic regulation system / B - PCB sensing system

1 - PCR product( made the 08/01/2013)digestion to degrade the backbone psB1C3

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