Notebook : July 12

summary

For fnr regulator system:

• Performed 4 transformations(3 terminator and 1 RBS+LacZ+terminator into competent cells).
• Design of oligo for amplifying AmiCP+RBS.

For PCBs sensor system:

• Had the promoter BphR1 clone 5 and 6, promoter BphR2 clone 3 and 4, promoter BphA1 clone 5 to 8 in stock.
• Plasmid DNA extraction from promoter BphR1 clone 5 and 6, promoter BphR2 clone 3 and 4, promoter BphA1 clone 5 to 8.

Lab work

A.aero/anaerobic regulation system:

• BioBrick RBS+LacZ+terminator in plasmid PSB1C3