Notebook : July 12

summary

For fnr regulator system:

• Performed 4 transformations(3 terminator and 1 RBS+LacZ+terminator into competent cells).
• Design of oligo for amplifying AmiCP+RBS.

For PCBs sensor system:

• Had the promoter BphR1 clone 5 and 6, promoter BphR2 clone 3 and 4, promoter BphA1 clone 5 to 8 in stock.
• Plasmid DNA extraction from promoter BphR1 clone 5 and 6, promoter BphR2 clone 3 and 4, promoter BphA1 clone 5 to 8.

Lab work

A.aero/anaerobic regulation system:

• BioBrick RBS+LacZ+terminator in plasmid PSB1C3

Transformation

We suspended the DNA with water from iGEM plate and performed several transformation.

• BBa_I732019: LacZ+RBS+terminator into plasmid BBa_I732950(ampicillin resitant).
• BBa_B0015: 2 terminators BBa_B0010+BBa_B0012(4F plate 3 kit 2013) into plasmid PSB1C3.
• BBa_B0017: 2 terminators BBa_B0010+BBa_B0010 into plasmid PSB1C3.
• Simple terminator BBa_B0010 into plasmid PSB1A2.

see protocol Tranformation.

B.PCBs sensor system:

The stock and extraction of promoter BphR1 clone 5 and 6, promoter BphR2 clone 3 and 4, promoter BphA1 clone 5 to 8 performed according to protocol. Please see protocol DNA extraction and sample stock.