Our main purpose is to provide a proof of concept for the use of RNA thermometers to regulate the expression of a transcription factor in artificial genetic circuits. We also provide guidelines for the standard characterization of other RNA thermometers and point at possible applications.
Our main purpose is to provide a proof of concept for the use of RNA thermometers to regulate the expression of a transcription factor in artificial genetic circuits. We also provide guidelines for the standard characterization of other RNA thermometers and point at possible applications.
We developed a model for the characterization of the dynamic behavior of RNA thermometers. We propose some of the parameters of our model to be used as quantitative points of reference to help us understand and reliably compare the behavior of RNA thermometers.