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Contents 1 Protocols 1.1 Repeat-Spacer Array Assembly 1.2 Assembling Parts from Oligonucleotides 1.3 (Multi) Site Directed Mutagenesis

Protocols

Repeat-Spacer Array Assembly

Assemble combinatorial libraries of repeat-spacer-repeat arrays using oligonucleotides

Assembling Parts from Oligonucleotides

Assemble short parts from annealed oligonucleotides

(Multi) Site Directed Mutagenesis

Site directed mutagenesis. Protocol is directly amenable simultaneously making distant mutations.