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Team:Paris Saclay/Notebook/July/12
From 2013.igem.org
Transformation
We suspended the DNA with water from iGEM plate and performed several transformation.
- BBa_I732019: LacZ+RBS+terminator into plasmid BBa_I732950(ampicillin resitant).
- BBa_B0015: 2 terminators BBa_B0010+BBa_B0012(4F plate 3 kit 2013) into plasmid PSB1C3.
- BBa_B0017: 2 terminators BBa_B0010+BBa_B0010 into plasmid PSB1C3.
- Simple terminator BBa_B0010 into plasmid PSB1A2.
Notebook : July 12
Lab work
'A - Aerobic/Anaerobic regulation system
Objective : obtaining Bba_K1155003, Bba_K1155007
1 -Transformation of Bba_I732019, Bba_B0015, Bba_B0017, Bba_B0010
Zhou
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Transformation of 07/10/13 of Bba_B0010 and Bba_I732019 didn't works. We will do it again. |
Protocol : Bacterial transformation
1 -Design of RBS-AmilCP oligos
Abdou, Anaïs
We used software gene manager to find the correct oligopeptides for amplification of RBS-Amil CP.
B - PCB sensor system
Objective : obtaining Bba_K1155001, Bba_K1155002 and BphR2
1 - Stock of clones 5, 6, 7, 8 for BphA1, clones 5, 6 for BphR1 and clones 3, 4 for BphR2
Zhou
Used quantities :
- Glycerol 85% : 425
- Culture : 1µL
2 - Extraction of clones 5, 6, 7, 8 for BphA1, clones 5, 6 for BphR1 and clones 3, 4 for BphR2 from DH5α
Abdou, Sheng
Protocol : High copy plamid extraction
3 - Streak of BphA1 and BphR2
Anaïs
We strek 25 colonies of 07/10/13 transformation of BphA1 and BphR2 in new solid culture.
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