/* Logo */
#logo h1 {
height: 26px;
text-indent: -4000px;
width: 187px;
padding:13px;
margin:0;}
#logo h1 a {display: block;height: 26px;}
/* Links */
a, a:visited {color:#3294a6;text-decoration:none;}
a:hover, .widget ul a:hover {color:#3294a6;text-decoration:underline;}
/* Theme Color */
#header-top, h3.deco span.inner, #footer, .post-format, #commentform #submit, .flex-direction-nav li a:hover, .overlay .caption span, #portfolio-sort a.active {background: #3294a6;}
.post-format {background-color: #3294a6;}
#portfolio-sort li a, #primary-nav ul li a:hover, #primary-nav ul li:hover, #primary-nav ul li.sfHover a, #primary-nav ul li.current-cat a, #primary-nav ul li.current_page_item a, #primary-nav ul li.current-menu-item a {color:#3294a6 !important;}
/* Clearfix */
.clearfix{*zoom:1;}.clearfix:before,.clearfix:after{display:table;content:"";}.clearfix:after{clear:both;}
/* Responsive Images */
img, object, embed {max-width: 100%;height: auto;}
img {-ms-interpolation-mode: bicubic;}
#map_canvas img, .map_canvas img {max-width: none!important;}
/* =Document Setup
-------------------------------------------------------------- */
html {background: #e6e6e6 url("https://static.igem.org/mediawiki/2013/1/1c/Scau_bg.gif") repeat;}
body {
font: 13px/24px 'Open Sans',Helvetica, Arial, sans-serif;
color: #666;
background: -moz-linear-gradient(top, rgba(0,0,0,0.05) 0%, rgba(0,0,0,0) 300px);
background: -webkit-gradient(linear, left top, left bottom, color-stop(0%,rgba(0,0,0,0.05)), color-stop(300px,rgba(0,0,0,0)));
background: -webkit-linear-gradient(top, rgba(0,0,0,0.05) 0%,rgba(0,0,0,0) 300px);
background: -o-linear-gradient(top, rgba(0,0,0,0.05) 0%,rgba(0,0,0,0) 300px);
background: -ms-linear-gradient(top, rgba(0,0,0,0.05) 0%,rgba(0,0,0,0) 300px);
background: linear-gradient(top, rgba(0,0,0,0.05) 0%,rgba(0,0,0,0) 300px);
text-align: justify;
}
a {
text-decoration:none;
outline:0;
-webkit-transition-property:background color;
-webkit-transition-duration:.12s;
-webkit-transition-timing-function:ease-out;
-moz-transition-property:background color;
-moz-transition-duration:.12s;
-moz-transition-timing-function:ease-out;
-o-transition-property:background color;
-o-transition-duration:.12s;
-o-transition-timing-function:ease-out;
transition-property:background color;
transition-duration:.12s;
transition-timing-function:ease-out;}
a:hover {color:#222;cursor:pointer;}
/* Headings */
h1, h2, h3, h4, h5, h6 {font-weight:bold; margin: 0 0 10px; color:#3a3a40;}
.entry-content h1, .entry-content h2, .entry-content h3, .entry-content h4, .entry-content h5, .entry-content h6 {margin: 0 0 20px;}
.entry-content h1 {font-size: 22px;line-height: 28px;}
.entry-content h2 {font-size: 18px;line-height: 26px;}
.entry-content h3 {font-size: 16px;line-height: 26px;}
.entry-content h4 {font-size: 14px;line-height: 24px;}
.entry-content h5 {font-size: 13px;line-height: 22px;}
.entry-content h6 {font-size: 12px;line-height: 20px;}
/* Page Structure */
#site-container {
margin: 0 auto;
background:#fff;
width: 960px;
padding:0 25px;
box-shadow:0 0 10px rgba(0,0,0,0.1);}
#content1 {float:left;width: 670px;margin:0 45px 0 0;}
#content1.fullwidth {width:100%;float:none;margin:0;}
#sidebar {float:left;width:195px;font-size:12px;margin:10px 0 0;}
#primary {padding:0 25px;}
/* Header */
#header {background: url("https://static.igem.org/mediawiki/2013/a/ab/SCAU-Doubleborder.gif") repeat-x bottom;padding:10px 25px;margin:0 0 20px;}
#header #logo {float:left;padding:25px 0 30px;margin:0 0 -2px; float:left;}
#header #logo h1 {margin:0;font-size: 32px;}
#header #logo, #header #logo a {
font-size: 32px;
color:#1a1a1a;
text-decoration:none;
text-transform:uppercase;
font-weight: bold;
letter-spacing: -1px;}
#header #logo img {display:block;}
#header-top {
color:#fff;
padding:5px 10px;
font-size:12px;}
#header-top .left {float:left;}
/* Page Header */
#page-header {background: url("https://static.igem.org/mediawiki/2013/a/ab/SCAU-Doubleborder.gif") repeat-x bottom; margin:0 0 30px; padding:0 25px 20px; position:relative;}
#page-header h1, #page-header strong {font-size: 26px;margin:0 0 5px;letter-spacing:-1px;color:#262626;}
#page-header p {
margin:0;
color: #8C8C8C;
font-size: 14px;}
#page-header .navigation {float:right;}
/* Footer */
#footer {
padding: 25px;
margin: 15px 0 0;
color: #fff;
font-size: 12px;
}
#footer a {color:#fff;border-bottom:1px dotted #fff}
#footer a:hover {border-bottom:none;}
#footer-widgets {list-style:none;padding:0;margin:0 -25px 0 0;}
#footer-widgets .widget {float:left;width:286px;margin:0 25px 0 0;}
#footer-widgets .widget h4 {
background: none;
color: #fff;
border-bottom: none;
padding: 0;
margin-top: 0;
margin-right: 0;
margin-bottom: 10px;
margin-left: 0;
}
#footer-widgets .widget .div {
background:#fff;
height:1px;
width:50px;
display:block;
margin:0 0 15px;}
#footer-bottom {
background:#1a1a1a;
padding:20px 25px;
margin:0;
line-height: 10px;
color:#fafafa;
font-size:11px;}
#footer-bottom #footer-nav {float:left;}
#footer-nav ul {margin:2px 0 0;padding:0;list-style:none;}
#footer-nav li {
float:left;
margin:0 10px 0 0;
padding:0 10px 0 0;
border-right: 1px dotted #8c8e91;
line-height: 5px;}
#footer-nav li:last-child {border-right:none;}
#footer-nav li a {border-bottom:none;color:#fff;}
#footer-bottom a {color:#fff;font-size:12px;border-bottom:1px dotted #fafafa;}
#footer-bottom a:hover {border-bottom:1px solid #fff;text-decoration:none;}
#footer-bottom #copyright {float:right;font-size:11px;}
/* =Navigation & Menus */
#primary-nav { float:right;margin: 30px 0 0;padding:0;}
#primary-nav ul {list-style: none outside none;margin: 0;padding: 0;}
#primary-nav ul a {
display: block;
position: relative;
color: #3A3A40;
line-height: 18px;
text-decoration:none;
text-transform:uppercase;
height: 32px;}
#primary-nav ul a.sf-with-ul {padding-right:15px;}
#primary-nav ul li {float: left;position: relative;z-index: 40;margin-left: 30px;}
#primary-nav ul li:hover {visibility: inherit;}
#primary-nav ul li:hover ul, #primary-nav ul li.sfHover ul {left: -1px;top: 20px;z-index: 99;}
#primary-nav ul li:hover li ul, #primary-nav ul li.sfHover li ul, #primary-nav ul li li:hover li ul, #primary-nav ul li li.sfHover li ul, #primary-nav ul li li li:hover li ul, #primary-nav ul li li li.sfHover li ul {top: -9999em;}
#primary-nav ul li li:hover ul, #primary-nav ul li li.sfHover ul, #primary-nav ul li li li:hover ul, #primary-nav ul li li li.sfHover ul, #primary-nav ul li li li li:hover ul, #primary-nav ul li li li li.sfHover ul {left: 180px;top: -12px;}
#primary-nav ul li a:hover, #primary-nav ul li:hover, #primary-nav ul li.sfHover a, #primary-nav ul li.current-cat a, #primary-nav ul li.current_page_item a, #primary-nav ul li.current-menu-item a {text-decoration: none;}
#primary-nav ul ul {
background: #fff;
border: 1px solid #E0E0E0;
box-shadow: 2px 2px 0 rgba(0, 0, 0, 0.05);
margin: 10px 0 0;
padding: 4px 0;
position: absolute;
top: -9999em;
width: 180px;
border-radius: 1px;
-moz-border-radius: 1px;
-webkit-border-radius: 1px;}
#primary-nav ul ul li {background: none;float: none;width: 100%;margin:0;}
#primary-nav ul ul li:last-child {border-bottom: medium none;}
#primary-nav ul ul li a {
font-size: 11px;
font-weight: normal;
color:#808080;
display: block;
padding: 8px 4px;
margin: 0 10px;
height: 18px;
border-bottom: 1px dotted #ccc;}
#primary-nav ul ul li:last-child a {border-bottom:0;}
#primary-nav .sf-sub-indicator {
display:inline;
padding:0;
margin:0;
border:none;
position:absolute;
right:0;
top:0; }
#primary-nav ul ul .sf-sub-indicator {right: 10px;top: 9px;}
#primary-nav ul li.sfHover ul a {color: #444 !important;}
#primary-nav ul li.sfHover ul a:hover {color: #444 !important;}
#primary-nav select {display:none;}
/* =Homepage */
#callout {padding:0;float:left;margin:0 30px 0 0;width:300px;}
#callout h2 {font-size:38px;font-weight:bold;line-height:38px;margin:0 0 10px;text-transform:uppercase;letter-spacing:-2px;color:#212126;}
#callout h3 {
font-size:18px;
font-weight:normal;
color:#8c8c8c;
font-style:italic;
font-size:center;}
#hpslider {background: url("../images/loading.gif") no-repeat center center;min-height: 250px;margin:10px 0 25px;float:left; width:580px;}
.home .st_portfolio {margin-bottom:15px;}
.home .post {background:none;margin-bottom:20px; }
.home .st_portfolio .entry-thumb {margin:0 0 5px;}
.home .post .entry-thumb {margin:0 0 10px;}
#home-portfolio ul, #home-blog ul, #home-blocks ul {list-style:none;padding:0;margin: 0 -15px 20px 0;}
#home-portfolio li {float:left;margin:0 15px 10px 0;width:216px;text-align:center;}
#home-portfolio .entry-content p {margin:0;}
#home-portfolio .entry-title, #home-blog .entry-title {margin:0;}
#home-portfolio .entry-title, #home-portfolio .entry-title a, #home-blog .entry-title, #home-blog .entry-title a {font-weight:bold;color: #3A3A40;font-size: 14px;line-height: 18px;}
#home-blog li, #home-blocks li {
float:left;
margin:0 15px 15px 0;
padding:0;
width:216px;
font-size:12px;
border:none;}
#home-blog .entry-title { margin: 0 0 10px;}
#home-blog .entry-thumb-placeholder {height: 172px;margin: 0 0 10px;width: 100%;}
h3.deco {
display:block;
background: url("../images/doubleborder.gif") repeat-x center;
margin:0 -25px 20px;
text-align:center;
text-transform:uppercase;
font-weight:normal;
clear: both;
font-size: 13px;}
h3.deco span.outer {background:#fff;}
h3.deco span.inner {
padding:2px 10px;
margin:0 10px;
color:#fff;}
/* =Flexslider */
.flex-container a:active, .flexslider a:active, .flex-container a:focus, .flexslider a:focus {outline: none;}
.slides, .flex-control-nav, .flex-direction-nav {margin: 0; padding: 0; list-style: none}
/* FlexSlider Necessary Styles */
.flexslider {width: 100%; margin: 0; padding: 0;}
.flexslider .slides > li {display: none; -webkit-backface-visibility: hidden;}
.flexslider .slides img {max-width: 100%; display: block;border-radius:2px;-moz-border-radius:2px;-webkit-border-radius:2px;}
.flex-pauseplay span {text-transform: capitalize;}
.slides:after {content: "."; display: block; clear: both; visibility: hidden; line-height: 0; height: 0;}
html[xmlns] .slides {display: block;}
* html .slides {height: 1%;}
/* No JavaScript Fallback */
.no-js .slides > li:first-child {display: block;}
/* FlexSlider Styling */
.flexslider {background: #fff; position: relative; zoom: 1;margin: 0 0 20px;}
.flexslider .slides {zoom: 1;}
.flexslider .slides > li {position: relative;}
.flex-container {zoom: 1; position: relative;}
/* Caption style */
.flex-caption {background:#232326; padding: 10px; margin: 0; position: absolute; left: 20px; bottom: 20px; color: #fafafa; font-size: 13px; line-height: 18px;max-width: 55%;}
/* Direction Nav */
.flex-direction-nav { height: 0; position:absolute; bottom: 0; right:20px; }
.flex-direction-nav li a {display: block; position: absolute; bottom: 20px; cursor: pointer; background:#232326; color:#fafafa; font-family:"Trebuchet MS", Arial, Helvetica, sans-serif; padding: 3px 10px; text-align:center;border-radius:2px;-moz-border-radius:2px;-webkit-border-radius:2px; }
.flex-direction-nav li a:hover {text-decoration:none;}
.flex-direction-nav li .next {right: 0;}
.flex-direction-nav li .prev {right: 30px;}
.flex-direction-nav li .disabled {opacity: .3; filter:alpha(opacity=30); cursor: default;}
/* Control Nav */
.flex-control-nav {position: absolute; bottom: 20px;text-align:right;right:90px;}
.flex-control-nav li {margin: 0 0 0 5px; display: inline-block; zoom: 1; *display: inline;}
.flex-control-nav li:first-child {margin: 0;}
.flex-control-nav li a {width: 13px; height: 13px; display: block; background: url("../images/flex-control-nav.png") no-repeat; cursor: pointer; text-indent: 9999px;overflow: hidden; transition:none;-moz-transition:none;}
.flex-control-nav li a:hover {background-position: 0 -13px;}
.flex-control-nav li a.active {background-position: 0 -26px; cursor: default;}
/* Flexslider - Gallery Post Format */
.format-gallery .flex-direction-nav li a {bottom:10px;}
.format-gallery .flex-direction-nav {right:10px;}
Overview
Organophosphorus pesticides (OP), which are highly toxic, are still used in some developing countries. Many terrestrial and aquatic ecosystems across the world are contaminated with OP. Residues of OP in food are also often reported
This year, our goal is to construct a system that can sensing and degrade organophosphorus pesticides.
The bacterial organophosphorus hydrolase enzyme hydrolyzes and detoxifies a broad range of toxic organophosphate pesticides. In the first part, we need to use these enzymes to degrade parathion into PNP.
The second part is a biosensor of the hydrolysis product P-Nitrophenol (PNP). There is no operon that can react to parathion. We foucus on constructing a PNP sensor, which can reflect the existence of parathion.
We also design a PNP degradation system. We use enzymes NphA1 and NphA2 to degrade PNP, but fail. We find another pathway to degrade PNP in 5 steps with 4 enzymes in Rhodococcus sp. Strain PN1.
With the public increasingly concerned about biosafety, we conceived a suicide gene circuit to ensure these recombinanational bacteria can only survive in laboratories and not cause horizontal gene transfer.
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