Team:Paris Saclay/Notebook/July/18

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Contents

1 Notebook : July 18
- 1.1 Lab work
  - 1.1.1 A - Aerobic/Anaerobic regulation system
    - 1.1.1.1 Objective : obtaining Bba_K1155003, Bba_K1155007
    - 1.1.1.2 1 - PCR of Bba_B0015, Bba_B0017 digested by EcoRI/PstI

Notebook : July 18

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining Bba_K1155003, Bba_K1155007

1 - PCR of Bba_B0015, Bba_B0017 digested by EcoRI/PstI

Abdou, Anaïs

Used quantities :

Buffer Dream Taq : 5µL
dNTP : 1µL
Oligo VF : 2µL
Oligo VR : 2µL
H2O : 37.75µL
DNA : 2µL
Dream Taq : 0.25µL

PCR program :

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