66.5 ml distilled water
10 ml 10x S-base
2.5 ml 20% (w/v) glucose
5 ml 0.1% (w/v) L-tryptophan
1 ml 2% (w/v) casein
5 ml 10% (w/v) yeast extract (Difco)
10 ml 8% (w/v) arginine
0.4% histidine
autoclave all components separately
tryptophan solution: sterile filtration
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