Team:NYMU-Taipei/Experiment/Wet Lab

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National Yang Ming University


Contents

Inhibition of Spore Germination Testing---Mannosidase Function Assay

Nymu 流程圖.png

We define the germination degree as follows:

1 point:A spore begins its budding reproduction.
2 points:A new spore is ccompletely germinanated from the old one.
3 points:A cluster of duplicated spores is formed.
4 points:A Spore extrude a polar filament.

And so far we have accomplished experiments of the first two groups, discovering that spores underwent the germination procesure got much more points than the control group. Next we will compare the points of group 3 and group 4 to see if Bee. coli can inhibit the germination of N. ceranae.

N. ceranae Experiment Protocols

Extraction of N. ceranae Spores from Bees

Extraction.png

Purification of N. ceranae Spores

Nymu-Purification.png

N. ceranae Genomic DNA Extraction

Nymu-Genomic.png

Germination of N. ceranae Spores

Nymu-Germination.PNG

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