Team:Hong Kong HKUST/Project/module3
From 2013.igem.org
Revision as of 16:07, 27 September 2013 by CeciliaShi (Talk | contribs)
![](https://static.igem.org/mediawiki/igem.org/c/c7/BANNER1_%281%29.png)
-
Protein Trafficking
- Overview
- Mechanism of MLS
- Linkage to Project
- Reference
-
Modules
- FA Quantification & Cell Viability
- FA Sensing Mechanism
- Protein Trafficking
- Glyoxylate Shunt
Protein Trafficking
Overview
In nature, eukaryotic cell mitochondria usually have their protein encoded by gene in nucleus and produced from cytosol. In this process, MLS will act as signal peptide to target the protein into mitochondria.In our module, we will construct the MLS BioBrick K1119001, and characterize it quantitatively.
Mechanism of MLS
MLS is attached to the N-terminal of enzyme, and bind to the receptor protein on mitochondrial membrane, and diffuse to contact site where inner and outer membrane fuse, then bring the ACE enzyme into mitochondria. Afterward, it is be cleaved, leaving the enzyme in mitochondria.![](https://static.igem.org/mediawiki/2013/8/87/MLS_mechanism.png)