"
Protein Trafficking
Responsible for:
Target ACE proteins into mitochondria
Parts submitted: BBa_K1119000, BBa_K1119001 & BBa_K1119009
Team:Hong Kong HKUST/modules
From 2013.igem.org
Project Outline
Our ultimate goal is to build a ‘smart’ glyoxylate shunt that burns extra fatty acid there is a surplus of energy. To achieve this, we would have to first build the shunt itself in mammalian cells, which consists of two bacterial enzymes, isocitrate lyase (AceA) and malate synthase (AceB). These two glyoxylate enzymes were tagged with localization signal peptide, so they could be directed to the mitochondria and act on the citric acid cycle, eventually increasing fatty acid uptake. The two enzymes were initially driven by constitutive promoters (CMV and EF-1alpha promoters), which put the shunt in a constantly “ON” state and burns calories regardless of the energy profile. To improve this, we are developing fatty acid responsive promoters, which, when used to regulate the glyoxylate enzymes, should dispense energy only when it is in excess. Hover your mouse and click on the images below to learn more about each modules!
Cell Viability & Fatty Acid Quantification
Responsible for:
Measuring cell viability at different fatty acid concentration & measure fatty acid uptake rate
Parts submitted: -
Fatty Acid Sensing Mechanism
Responsible for:
Introduce inducible system that allows tunable fatty acid uptake by sensing fatty acid concentration
Parts submitted: -
Glyoxylate Shunt
Responsible for:
Introduce glyoxylate enzymes responsible for the shunt
Parts submitted:BBa_K1119002, BBa_K1119003, BBa_K1119004, BBa_K1119006 & BBa_K1119008
