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Team:NYMU-Taipei/Safety
The chassis organisms we use for this project
| Species | Strain no/name | Risk Group | Disease risk to humans? If so, which disease | |
|---|---|---|---|---|
| 1 | E. coli (K 12) | DH5 alpha | [http://www.absa.org/riskgroups/bacteriasearch.php?genus=&species=coli 1] | Yes. May cause irritation to skin, eyes, and respiratory tract, may affect kidneys. However, E. coli K-12 is not considered a human or animal pathogen nor is it toxicogenic. Any concerns for E. coli K-12 in terms of health considerations are mitigated by its poor ability to colonize the colon and establish infections. |
| 2 | E. coli (K 12) | MG1655 | [http://www.absa.org/riskgroups/bacteriasearch.php?genus=&species=coli 1] | Same as above |
List and description of all new or modified coding regions we used
| Part number | Where did us get the physical DNA for this part | What species does this part originally come from? | What is the Risk Group of the species? | What is the function of this part, in its parent species? | |
|---|---|---|---|---|---|
| 1 | BBa_K1104100 | Bioresource Collection and Research Center | Escherichia coli K-12 substr. MG1655 | 1 | An enzyme involved in the cleavage of mannose |
| 2 | BBa_K1104200 | Bioresource Collection and Research Center | Escherichia coli K-12 substr. MG1655 | 1 | DNA-binding transcriptional dual regulator |
| 3 | BBa_K1104300 | National Ilan University | Apis mellifera | 1 | antimicrobial peptide |
| 4 | BBa_K1104301 | National Ilan University | Apis mellifera | 1 | antimicrobial peptide |
| 5 | BBa_K1104400 | National Taipei University of Technology | Zymomonas mobilis | 1 | alcohol dehydrogenase |
| 6 | BBa_K1104401 | National Taipei University of Technology | Zymomonas mobilis | 1 | pyruvate decarboxylase |
Do the biological materials used in our lab work pose any of the following risks?
Risks to the safety and health of team members or others working in the lab? All the biological materials used in our lab are safe to the workers in the lab. Every species we worked with belongs to BL1. Risks to the safety and health of the general public, if released by design or by accident? Every species we worked with belongs to BL1. They do no harm to human. Risks to the environment, if released by design or by accident? Every species we worked with belongs to BL1. They do no harm to the environment. In addition, we have designed a lysis system to ensure our genetic modified E. coli will not survive in the natural environment. Risks to security through malicious misuse by individuals, groups, or countries? All the biological materials used in our lab are safe, and all the products of the coding sequences are innocuous to human, so the risk of malicious misuse is low.
