Team:BYU Provo/Notebook/Cholera - Enzymes/Protocols

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Cholera - Enzyme Laboratory Protocols



Cholera Enzyme
March-April
May-June
July-August
September
Protocols

Cholera-Enzyme Protocols



Phusion PCR (50ul reaction)


For each sample add: 

35 ul ddH2O
10 ul 5X Phusion buffer
1.5 ul 10mM dNTP's
1 ul each Primer (50 uM stock)
1 ul appropriate diluted template DNA
0.5 ul Phusion Polymerase


Taq polymerase PCR (50ul reaction)


For each sample add: 

40 ul ddH2O
5 ul 10X TAQ buffer
1.5 ul 10 mM dNTP's
1 ul each Primer (50 uM stock)
1 ul appropriate diluted template DNA
0.5 ul TAQ Polymerase


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