Team:UC Davis/Parts
From 2013.igem.org
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Parts
The following parts were assembled throughout the development of this project:Name | Type | Description | Designer | Length |
---|---|---|---|---|
BBa_K1212011 | Composite | pBAD+Riboswitch 2+TAL1 | Amy Soon | 2612 |
BBa_K1212012 | Composite | pBAD+Riboswitch 2+TAL8 | Amy Soon | 2616 |
BBa_K1212013 | Composite | pTet+TBS 1+RBS+GFP+T | Aura Ferreiro | 1003 |
BBa_K1212014 | Composite | pBAD+Riboswitch 1+TAL 1 | Amy Soon | 2616 |
BBa_K1212015 | Composite | pBAD+Riboswitch 1+TAL 8 | Amy Soon | 2620 |
BBa_K1212016 | Composite | pTet+TBS 2+RBS+GFP | Amy Soon | 1003 |
Name | Type | Description | Designer | Length |
---|---|---|---|---|
BBa_K1212002 | Plasmid_Backbone | Golden Gate Compatible pSB3K3 | Alex Neckelmann | 2750 |
BBa_K1212006 | Composite | pBAD+Golden Gate Compatible GFP | Aura Ferreiro | 1052 |
BBa_K1212009 | Composite | pBAD+Riboswitch 2+GFP+T | Amy Soon | 1086 |
BBa_K1212010 | Composite | pBAD+Riboswitch 1+GFP+T | Amy Soon | 1090 |
Our best characterized parts are our RiboTAL constructs, which consist of parts BBa_K1212011-BBa_K1212016. Among these parts, most of characterization was focused on the riboswitch+TAL constructs (BBa_K1212011, BBa_K1212012, BBa_K1212014, BBa_K1212015), as the other parts were simply pTet+GFP, which are already well characterized parts, with some extra base pairs added for our TAL binding sites (BBa_K1212013, BBa_K1212016). Both our pSB3K3 backbone (BBa_K1212002) and our GFP (BBa_K1212006) with their BsaI sites fixed were used in our RiboTAL constructs and functioned as expected but did not need separate characterization.
![]() Project BackgroundLearn about how we combine riboswitches and TALs into robust orthogonal mechanisms for inducible repression. |
![]() ResultsCheck out the results of our experiments. |
![]() Human PracticesTake a look at how we designed a new database for better raw data characterization of Biobricks. |
![]() Judging CriteriaHere's the criteria that we met for this year's team. |