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Team:USTC CHINA
From 2013.igem.org
Motivation
We hope to develop a fresh-expression transdermal vaccination.
We hope everyone in this world could equally enjoy the benefit of medical progress.
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We hope everyone in this world could equally enjoy the benefit of medical progress.
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Design of Project
With the support of TD-1, a special polypeptide which can greatly facilitate macromolecule transdermal delivery through intact skin, we construct three different fuctional engineered bacillus subtilis; besides, we design a reporter system, including a kill switch, in the fouth type B.subtilis to improve usability and ensure biosafety.
ANTIGEN
As the core of our in-situ system,
TD1-antigen expresser is designed
and will share a great percentage of
the total bacteria in our "band-aid".
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As the core of our in-situ system,
TD1-antigen expresser is designed
and will share a great percentage of
the total bacteria in our "band-aid".
more
LTB
As one kind of our adjuvant expresser,
TD1-LTB is used to enhance the antigenicity.
For LTB has so many advantages, it's a wild using adjuvant for most of exsiting injected vaccine.
more
As one kind of our adjuvant expresser,
TD1-LTB is used to enhance the antigenicity.
For LTB has so many advantages, it's a wild using adjuvant for most of exsiting injected vaccine.
more
TNFα
TNFαis a chemotactic factor that recruits Langerhans Cells, antigen presenting cells beneath the skin barrier. The TNFα expresser is responsible for immigrating LC and triggering more intense immune responses.
more
TNFαis a chemotactic factor that recruits Langerhans Cells, antigen presenting cells beneath the skin barrier. The TNFα expresser is responsible for immigrating LC and triggering more intense immune responses.
more
REPORTER
Reporter notifies users whether the status of vaccine patch is all right and when they can stick the patches to arms . Further more killing switch is conveyed in them as a biosafety guard.
more
Reporter notifies users whether the status of vaccine patch is all right and when they can stick the patches to arms . Further more killing switch is conveyed in them as a biosafety guard.
more
Experimental Measurement
Construction of Vectors
We took E.coli and the PET vector as the positive control and realized our ideas in the B.subtilis expression system based on PHT43 shuttle vector.
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We took E.coli and the PET vector as the positive control and realized our ideas in the B.subtilis expression system based on PHT43 shuttle vector.
more
Tests on fusion protein
To achieve fresh expression ultimately, we used purified protein to obtain accurate data and verified design.
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To achieve fresh expression ultimately, we used purified protein to obtain accurate data and verified design.
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Immunogenicity experiments
The most prominent character of 2013USTC iGEM team is that we have not only achieved a series of molecule experiments but also conducted mice experiments, including in vitro transdermal experiments and mice experiments
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The most prominent character of 2013USTC iGEM team is that we have not only achieved a series of molecule experiments but also conducted mice experiments, including in vitro transdermal experiments and mice experiments
more
Thanks to the sponsors
Deutsche Bank, China
School of Life Sciences, USTC
USTCIF
School of the Gifted Young, USTC
School of physics, USTC
Teaching Affairs Office of USTC
