Team:Paris Saclay/Notebook/August/20

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Notebook : August 20

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining Bba_K1155004, Bba_K1155005, Bba_K1155006

1 - Sequences analysis

Damir, XiaoJing

Sequencies were good. We obtain : Bba_K1155004, Bba_K1155005, Bba_K1155006.

A - Aerobic/Anaerobic regulation system / B - PCB sensor system

Objective : obtaining FNR and BphR2 proteins

1 - PCR of BphR2 Part I

Damir

Used quantities :

  • Oligo 54F : 2µL
  • Oligo 55R : 2µL
  • DNA : 1µL
  • Buffer Phusion : 10µL
  • dNTP : 1µL
  • Phusion : 1µL
  • DMS9 : 2µL ??????????????????????????????????
  • H2O : 31µL

PCR program :

Pstest.jpg

2 - Electrophoresis of PCR products : BphR2 Part I

Nadia

[[]]
  • Well 1 : 6µL DNA Ladder
  • Well 2 : 40µL BphR2 PartI+8µl of 6X loading dye
  • Gel : 0.8%

We obtain a frangment at the right size. We can purify it.

2 - Gel purification of PCR products : BphR2 Part I

Damir, Nadia

Protocol : [http://www.mn-net.com/tabid/1452/default.aspx Gel purification ]

Nanodrop :

  • BphR2 Part I: .........

We lost our fragment. We will do the PCR again.


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