Team:INSA Toulouse/contenu/lab practice/notebook/calendar/logic gates

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Calendar

Logic Gates Characterization

July 2013

  • Week 6 (15-21 July)
    15/07
    PCR to invert RFP and rbs.LuxI with the restriction site ClaI BamHI

    16/07
    Purification of the PCR and verification by gel.

    19/07
    Assembling of rbs.LuxI and cI for the final system with the AND1 gate.
    Arrival of the synthesis.

    20/07
    Verification of the clonage.
    Assembling the synthesis parts (the 4 gates) into pSB1C3.

  • Week 7 (22-28 July)
    22/07
    Assembling XOR1 with FimE, LuxI with cI

August 2013

September 2013

  • Week 1 (02-08 Sept)

    • 06/09
    Cloning AND2 and XOR2 with RFPinv

    08/09
    Verfication of the cloning, AND2.RFPinv ok

  • Week 1 (09-15 Sept)

    • 10/09
    Cloning AND2.RFPinv with PolT7 and XOR2 with RFPinv

    11/09
    In fusion to have XOR2 with RFPinv because cloning is not working

    13/09
    XOR2.RFPinv with in fusion OK

    14/09
    Cloning XOR2.RFPinv and AND2.RFPinv with the good PolT7 (with promoter)

    15/09
    XOR2.RFPinv and AND2.RFPinv with PolT7 ok for characterisation

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