Team:Freiburg/plasmids

Revision as of 16:56, 30 September 2013 by PhilippS (Talk | contribs)

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oligo list
oligo name	Sequence 5'-3'	description
oIG0000	TGAGCAAGAGGTAAGGG	Sequencing Primer
oIG0001	AAACTGGTGGACAGCAC	Sequencing Primer
oIG0001_new	GGACAGCACCGACAAGG	Sequencing Primer
oIG0002	AGCTGCCTGAGAAGTAC	Sequencing Primer
oIG0003	TGGACCTGCTGTTCAAG	Sequencing Primer
oIG0004	ACGAGCTCGTGAAAGTG	Sequencing Primer
oIG0005	AGAATGACAAGCTGATCCG	Sequencing Primer
oIG0006	TGGCCTATTCTGTGCTG	Sequencing Primer
oIG0007	CAGGCCGAGAATATCATCC	Sequencing Primer
oIG0008	CAAACAACAGATGGCTGGC	Sequencing Primer
oIG0009	CTCCGATCAGGTTCTTC	Sequencing Primer
oIG0010	GACAAGAAGTACAGCATCG	Sequencing Primer
oIG0011	CCCGTAATGCAGAAGAAG	Sequencing Primer
oIG0012	GAAGCGGAGCTACTAAC	Sequencing Primer
oIG0013	GACAATGCGATGCAATTTC	Sequencing Primer
oIG0014	CTTGGTCACCTTCAGCTTG	Sequencing Primer
oIG0015	TTTCTTGGGTAGTTTGCAG	Sequencing Primer
oIG0016	ACCAAAATCAACGGGAC	Sequencing Primer
oIG0017	ACCAATGTGACCGAGTTCG	Sequencing Primer
oIG0018	GCCAGCCATCTGTTGTTTG	Sequencing Primer
oIG0019	AGAAGCGCGATCACATG	Sequencing Primer
oIG0020	CTAATTACAGCCCGGAGGA	Sequencing Primer
oIG0021	ACCAGAAAGTTAACTGG	Sequencing Primer (Bb Michael fwd)
oIG0022	TGGAATTCGCGGCCGCTTCTAGATGGCCGGCGACAAGAAGTACAGCATCGG	Start+RFC25 fv
oIG0023	CTGCAGCGGCCGCTACTAGTATTAACCGGTGTCGCCTCCCAGCTGAG	Ende+RFC25 rev
oIG0024	TCCGGCCTTATCCAGTTCGC	2742 (C-->A) rv
oIG0025	GCGAACTGGATAAGGCCGGATTCATCAAGAGACAGCTGGTGG	2742(C-->A) fv
oIG0026	TTGCAGGTAGTACAGGTACAGCTTCTCGTTCTGAAGCTGGGTGTTTTCCACGG	2415(G-->T)/2448 (G-->A!) rev
oIG0027	TGTACCTGTACTACCTGCAAAATGGGCGGGATATGTACGTG	2415(G-->T)/2448(G-->A!) fv
oIG0028	TGGCTCCGCCGTCAATGTATCCGGCGTAGCCGTTCTTG	1080(C-->A) rev
oIG0029	ATACATTGACGGCGGAGCC	1080(C-->A) fv
oIG0031	GGGCGAACTGCAAAAGGGAAACGAACTGGCCC	3648(C-->T)/3660(G-->A!) fv
oIG0032	TTCCCTTTTGCAGTTCGCCCGCAGAGGCCAGCATTCTCTTC	3648(C-->G)/3660(G-->A!) rev
oIG0033	AGGCAGCCCCGCCATTAAGAAGGGCATCCTGCAAACAGTGAAGGTGGTGGACGAGCTCGTGAAAGTGATGGGACGGCACAAGCCCGAGAAC	2181 (C-->A)/2214(G-->A!)/2253(C-->A) fv
oIG0034	TCTTAATGGCGGGGCTGCCTGCCAGATTGGCAATGTGCTC	2181(C-->A)/2214(G-->A!)/2253(C-->A)rev
oIG0035	TGGCCGAGGATGCCAAACTTCAGCTGAGCAAGGACACCTAC	789 (G-->T) fv
oIG0036	AGGTGTCCTTGCTCAGCTGAAGTTTGGCATCCTCGGCC	789(G-->C) rev
oIG0037	ACCGGTTAATACTAGTAGCGGCCGC	pSB1C3-RFC25_fw (AgeI, SpeI, NotI)
oIG0038	GCCGGCCATCTAGAAGCGGC	pSB1C3-RFC25_rev (NgoMIV, XbaI)
oIG6017	GAGTGCCACCTGACGTCTAAGAAAC	primerpBS1C3fw sequencing
oIG6018	CGCCTTTGAGTGAGCTGATACCGC	primerpBS1C3rv sequencing
oIG0039	ATGATTTCTGGAATTCGCGGCCGCTTCTAGATGGCCGGCGCGCGTACGAAAAACAATTACGGGTC	VP16 [25] for
oIG0040	TTTTGCCGGACTGCAGCGGCCGCTACTAGTATTAACCGGTCCCACCGTACTCGTCAATTCCAAGG	VP16 [25] rev
oIG0041	ATGATTTCTGGAATTCGCGGCCGCTTCTAGATGGCCGGCATGGATGCTAAGTCACTAACTGCCTGG	KRAB [25] for
oIG0042	TTTTGCCGGACTGCAGCGGCCGCTACTAGTATTAACCGGTCCAGAGATCATTCCTTGCCATTCCTTC	KRAB [25] rev
oIG0043	TTCTGGAATTCGCGGCCGCTTCTAGATGGCCGGCCTGTGGAATGTGTGTCAGTTAGGGTG	SV40 Promoter [25] fw
oIG0044	CGGACTGCAGCGGCCGCTACTAGTATTAACCGGTAGCTTTTTGCAAAAGCCTAGGCCTCC	SV40 Promoter [25] rev
oIG0045	CCGGCGCAGGCTCCACCGGTTAATA	Linker: Scar (Ala-Gly) - Ala-Gly-Ser - Scar (Thr-Gly) [25] fw
oIG0046	CTAGTATTAACCGGTGGAGCCTGCG	Linker: Scar (Ala-Gly) - Ala-Gly-Ser - Scar (Thr-Gly) [25] fw
oIG0047	CCGGCTACCCATACGATGTTCCAGATTACGCTACCGGTTAATA	HA-tag – fw primer
oIG0048	CTAGTATTAACCGGTAGCGTAATCTGGAACATCGTATGGGTAG	HA-tag rev primer
oIG0049	TGATTTCTGGAATTCGCGGCCGCTTCTAGATGGCCGGCGTGCCTTCTAGTTGCCAGCC	BGH-Terminator fv
oIG0050	CCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATTAACCGGTCATAGAGCCCACCGCATCC	BGH-Terminator rev
oIG0051	CCGGCGATAGTGCTGGTAGTGCTGGTAGTGCTGGTACCGGTTAATA	DSAGSAGSAG-Linker fv
oIG0052	CTAGTATTAACCGGTACCAGCACTACCAGCACTACCAGCACTATCG	DSAGSAGSAG-Linker rev
oIG0053	ATATCTTGTGGAAAGGACG	colony pcr pIG3010 crRNA locus for
oIG0054	TTCTGCAGAATTGGCGCAC	colony pcr pIG3010 crRNA locus rev
oIG0055	AACCGGTGCAGAATTGG
oIG0056	TTTTGCCGGACTGCAGCGGCCGCTACTAGTATTAACCGGTGCAGAATTGGCGCACGCGCTAG
oIG0057	AGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGTGCCTTCTAGTTGCCAGCC	BGH (RFC 10) fwd
oIG0058	CCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTACATAGAGCCCACCGCATCC	BGH (RFC 10) rev
oIG0059	AGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGCTGTGGAATGTGTGTCAGTTAGGGTG	SV40 (RFC 10) fwd
oIG0060	CCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTAAGCTTTTTGCAAAAGCCTAGGCCTCC	SV40 (RFC 10) rev
oIG1000	CTAGCGCGTGCGCCAATTC	pCBh-fwd_pX334a
oIG1001	GAAAGCTCTGAGGCACGATGGCGTCCACATCGTAGTCGGACAG	hSpCsn1-H840A-rev_pX334a
oIG1002	TGTCCGACTACGATGTGGACGCCATCGTGCCTCAGAGCTTTCTG	hSpCsn-H840A-fwd_pX334a
oIG1003	GTACGCGCGCGACCAGCACTACCAGCACTGTCGCCTCCCAGCTGAGAC	VP16-linker-hSpCsn-rev_pX334a
oIG1004	TGGGAGGCGACAGTGCTGGTAGTGCTGGTCGCGCGCGTACGAAAAACAATTAC	hSpCsn-linker-VP16-fwd_pKM018
oIG1005	GGAGCGGGTTGATGACGTCAGCGTTCGAATTGCGGCCGCGGCAGGATCATAATCAGCCATAC	pX334a-SV40pA-rev_pKM018
oIG1007	AAACGGAAGGGCCTGAGTCCGAGCAGAAGAAGAAGT	sticky end bsbI-EMXI Locus-sticky end bsbI-fwd_pX334a
oIG1008	TAAAACTTCTTCTTCTGCTCGGACTCAGGCCCTTCC	sticky end bsbI-EMXI Locus-sticky end bsbI-rev_pX334a
oIG1009	CGATATCAGGAGGAAGGGCCTGAGTCCGAGCAGAAGAAGAAGGGCTCTCGCGACCTGCAGGG	AatII-EcorV-emxIoperator-NruI-SbfI-NheI-fwd_pKM602
oIG1010	CTAGCCCTGCAGGTCGCGAGAGCCCTTCTTCTTCTGCTCGGACTCAGGCCCTTCCTCCTGATATCGACGT	AatII-EcorV-emxIoperator-NruI-SbfI-NheI-rev_pKM602
oIG1011	AAACTTAAAAGTCGGCTGGTAGCGGGGAGGATCGGT	human VEGFA locus 1_VZ-8_fwd_pKM600
oIG1012	TAAAACCGATCCTCCCCGCTACCAGCCGACTTTTAA	human VEGFA locus 1_VZ-8_rev_pKM600
oIG1013	AAACGTGTGCAGACGGCAGTCACTAGGGGGCGCTGT	human VEGFA locus 2_VZ-573_fwd_pKM600
oIG1014	TAAAACAGCGCCCCCTAGTGACTGCCGTCTGCACAC	human VEGFA locus 2_VZ-573_rev_pKM600
oIG1015	AAACGACCTGCTTTTGGGGGTGACCGCCGGAGCGGT	human VEGFA locus 3_VZ+434b_fwd_pKM600
oIG1016	TAAAACCGCTCCGGCGGTCACCCCCAAAAGCAGGTC	human VEGFA locus 3_VZ+434b_rev_pKM600
oIG1017	AAACGTGAGTGTGTGCGTGTGGGGTTGAGGGCGTGT	human VEGFA locus 4_VZ-475__fwd_pKM600
oIG1018	TAAAACACGCCCTCAACCCCACACGCACACACTCAC	human VEGFA locus 4_VZ-475_rev_pKM600
oIG1019	CGATATCAGGATTAAAAGTCGGCTGGTAGCGGGGAGGATCGCGGCTCTCGCGACCTGCAGGG	AatII-EcorV-VEGFA_VZ-8-NruI-SbfI-NheI-fwd
oIG1020	CTAGCCCTGCAGGTCGCGAGAGCCGCGATCCTCCCCGCTACCAGCCGACTTTTAATCCTGATATCGACGT	AatII-EcorV-emxIoperator-NruI-SbfI-NheI-rev
oIG1021	CGATATCAGGAGTGTGCAGACGGCAGTCACTAGGGGGCGCTCGGCTCTCGCGACCTGCAGGG	AatII-EcorV-VEGFA_ VZ-573-NruI-SbfI-NheI-fwd
oIG1022	CTAGCCCTGCAGGTCGCGAGAGCCGAGCGCCCCCTAGTGACTGCCGTCTGCACACTCCTGATATCGACGT	AatII-EcorV-VEGFA_ VZ-573-NruI-SbfI-NheI-rev
oIG1023	CGATATCAGGAGACCTGCTTTTGGGGGTGACCGCCGGAGCGCGGCTCTCGCGACCTGCAGGG	AatII-EcorV-VEGFA_ VZ+434b-NruI-SbfI-NheI-fwd
oIG1024	CTAGCCCTGCAGGTCGCGAGAGCCGCGCTCCGGCGGTCACCCCCAAAAGCAGGTCTCCTGATATCGACGT	AatII-EcorV-VEGFA_ VZ+434b-NruI-SbfI-NheI-rev
oIG1025	CGATATCAGGAGTGAGTGTGTGCGTGTGGGGTTGAGGGCGTAGGCTCTCGCGACCTGCAGGG	AatII-EcorV-VEGFA_ VZ-475-NruI-SbfI-NheI-fwd
oIG1026	CTAGCCCTGCAGGTCGCGAGAGCCTACGCCCTCAACCCCACACGCACACACTCACTCCTGATATCGACGT	AatII-EcorV-VEGFA_ VZ-475-NruI-SbfI-NheI-rev
oIG1027	GTTCTCCTCCTCAACTG	Sequencing Primer pKM602
oIG1028	GTAACTTGAAAGTATTTCGATTTCTTG	DR-fwd_pX334a
oIG1029	GCGGCCGCAATTCGAACG	H1Promotor-fwd_BBpX334a
oIG1030	CACAAGATATATAAAGCCAAGAAATC	U6 Promotor-rev_BBpX334a
oIG2000	CAGACCCCGTAGAAAAGATCAAAGGATC	Ori1-hU6-CAGenhancer-hSpCas9-fwd_pX334a
oIG2001	GAAAGCTCTGAGGCACGATAGCGTCCACATCGTAGTCGGAC	hSpCas9-H840A-rev_pX334a
oIG2002	GTCCGACTACGATGTGGACGCTATCGTGCCTCAGAGCTTTC	H840A-linkerGSAGSAG-KRAB-fwd_pX334a
oIG2003	CTTAGCATCCATGCCGGCGGAGCCTGCTGATCCGGGGCTGTCGCCTCCCAGC	H840A-linkerGSAGSAG-KRAB-rev_pX334a
oIG2004	GACAGCCCCGGATCAGCAGGCTCCGCCGGCATGGATGCTAAGTCACTAACTGCCTGGTC	linkerGSAGSAG-KRAB-bgHTerminator-fwd_pKM102
oIG2005	AGAAGGCACAGTCGAGGCTGATCGAAGCTTGGATCCTCACACCTTCC	linkerGSAGSAG-KRAB-bgHTerminator-rev_pKM102/pX334a
oIG2006	GGAAGGTGTGAGGATCCAAGCTTCGATCAGCCTCGACTGTGCCTTCTAG	bgHTerminator-PuroR/Backbone1-fwd_pKM102/pX334a
oIG2007	CACACCCGCCGCGCTTAATGCG	bgHTerminator-PuroR/Backbone1-rev_pX334a
oIG2008	GCCCTGTAGCGGCGCATTAAGCG	Backbone1-AmpR-Ori1-fwd_pX334a
oIG2009	GGATCTCAAGAAGATCCTTTGATCTTTTCTAC	Backbone1-AmpR-Ori1-rev_pX334a
oIG2010	AAACGGAAGGGCCTGAGTCCGAGCAGAAGAAGAAGT	sticky end BbsI-EMX1-sticky end BbsI-fwd_pX334
oIG2011	TAAAACTTCTTCTTCTGCTCGGACTCAGGCCCTTCC	sticky end BbsI-EMX1-sticky end BbsI-rev_pX334
oIG2012	CTAGGGAAGGGCCTGAGTCCGAGCAGAAGAAGAAGGG	sticky end NheI-EMX1-sticky end NheI-fwd_pKM006
oIG2013	CTAGCCCTTCTTCTTCTGCTCGGACTCAGGCCCTTCC	sticky end NheI-EMX1-sticky end NheI-rev_pKM006
oIG2014	AATTGGAAGGGCCTGAGTCCGAGCAGAAGAAGAAGGG	sticky end EcoRI-EMX1-sticky end EcoRI-fwd_pKM006
oIG2015	AATTCCCTTCTTCTTCTGCTCGGACTCAGGCCCTTCC	sticky end EcoRI-EMX1-sticky end EcoRI-rev_pKM006
oIG2016	AAACGGATCTGCACCACCGGCAAGCTGCCCGTGCCCGT	sticky end BbsI-pKM006 target_nt650-sticky end BbsI-fwd_pX334
oIG2017	TAAAACGGGCACGGGCAGCTTGCCGGTGGTGCAGATCC	sticky end BbsI-pKM006 target_nt650-sticky end BbsI-rev_pX334
oIG2018	AAACGGCACGACTTCTTCAAGTCCGCCATGCCCGAGT	sticky end BbsI-pKM006 target_nt750-sticky end BbsI-fwd_pX334
oIG2019	TAAAACTCGGGCATGGCGGACTTGAAGAAGTCGTGCC	sticky end BbsI-pKM006 target_nt750-sticky end BbsI-rev_pX334
oIG2020	AAACGGCGACACCCTGGTGAACCGCATCGAGCTGAAGT	sticky end BbsI-pKM006 target_nt850-sticky end BbsI-fwd_pX334
oIG2021	TAAAACTTCAGCTCGATGCGGTTCACCAGGGTGTCGCC	sticky end BbsI-pKM006 target_nt850-sticky end BbsI-rev_pX334
oIG2022	AAACGGCGTCTATATCACCGCCGACAAGCAGAAGAAGT	sticky end BbsI-pKM006 target_nt950-sticky end BbsI-fwd_pX334
oIG2023	TAAAACTTCTTCTGCTTGTCGGCGGTGATATAGACGCC	sticky end BbsI-pKM006 target_nt950-sticky end BbsI-rev_pX334
oIG2024	AAACGGAGAGCTCGTTTAGTGAACCGTCAGATCGCCGT	sticky end BbsI-pKM006 target_CMVmin1-sticky end BbsI-fwd_pX334
oIG2025	TAAAACGGCGATCTGACGGTTCACTAAACGAGCTCTCC	sticky end BbsI-pKM006 target_CMVmin1-sticky end BbsI-rev_pX334
oIG2026	AAACGGCACGCTGTTTTGACCTCCATAGAAGACACCGT	sticky end BbsI-pKM006 target_CMVmin2-sticky end BbsI-fwd_pX334
oIG2027	TAAAACGGTGTCTTCTATGGAGGTCAAAACAGCGTGCC	sticky end BbsI-pKM006 target_CMVmin2-sticky end BbsI-rev_pX334
oIG2028	CCGGTGCCACCATGTACCCATAC	hSpCas9-H840A-fwd_pX334a
oIG2029	CGTAATCTGGAACATCGTATGGGTACATG	Ori1-hU6-CAGenhancer-hSpCas9-H840A-rev_pX334a
oIG2030	AAACCAAGGTCGGAGTACTGTCCTCCGACACTAGGT	sticky end bsbI-UAS_Zebrafish_Locus_pos58-sticky end bsbI-fwd
oIG2031	TAAAACCTAGTGTCGGAGGACAGTACTCCGACCTTG	sticky end bsbI-UAS_Zebrafish_Locus_pos58-sticky end bsbI-rev
oIG2032	AAACGAAATTCGGTCCATCCGCGCGCGCAGAGTGGT	sticky end bsbI-Bmp2b_Zebrafish_Locus_pos55-sticky end bsbI-fwd
oIG2033	TAAAACCACTCTGCGCGCGCGGATGGACCGAATTTC	sticky end bsbI-Bmp2b_Zebrafish_Locus_pos55-sticky end bsbI-rev
oIG2034	AAACAACTCCTCCATGCATATGTTAATGCGCCGCGT	sticky end bsbI-Pouf51-VOX_Zebrafish_Locus_pos125-sticky end bsbI-fwd
oIG2035	TAAAACGCGGCGCATTAACATATGCATGGAGGAGTT	sticky end bsbI-Pouf51-VOX_Zebrafish_Locus_pos125-sticky end bsbI-rev
oIG2036	TAATACGACTCACTATAGGGACGAAACACCGGGTTTTAGAGC	crRNA with T7 site (forward)
oIG2037	CACGCGCTAGAAAAAGTTTTGGGACC	crRNA (reverse)
oIG2038	TAATACGACTCACTATAGGGACCGGTGCCACCATGTACC	Cas9 with T7 site and Kozak-sequence (forward)
oIG2039	AATTGCGGCCGCTCCCCAGC	bGH Terminator (reverse)
oIG2040	GCAATAGCATCACAAATTTCACAAATAAAGC	SV40 Terminator (reverse)
oIG2041	TAATACGACTCACTATAGGGGTTGGAACCATTCAAAACAGCATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGCTTTTTGGCG	tracrRNA with T7 site (forward)
oIG2042	CGCCAAAAAGCACCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATGCTGTTTTGAATGGTTCCAACCCCTATAGTGAGTCGTATTA	tracrRNA (reverse)
oIG2043	AAACGATGCGGTTTTGGCAGTACATCAATGGGCGGT	sticky end BbsI-CMV target of pIG6000-sticky end BbsI-fwd
oIG2044	TAAAACCGCCCATTGATGTACTGCCAAAACCGCATC	sticky end BbsI-CMV target of pIG6000-sticky end BbsI-rev
oIG3001	GGCAGCGCCGGCTCCGGCAGCGCCTCCGGCATGGTGAGCAAGGGCGAGGAGG	Linker1-mCherry_forw_pMZ333
oIG3002	TCTGGATCGAAGCTTGGGCTGCAGGTCGACTCTAGATCACTTGTACAGCTCGTCCATGCCGC	mCherry-Bb_rev_pMZ333
oIG3003	TGTCTTTTATTTCAGGTCCCGGATCGAATTGCGGCCGCAGGAGGCGCCACCATGTACCCATACGATGTTCCAGATTACGCTGACAAGAAGTACAGCATCGGCCTG	Bb-HA-Cas9_forw_pX334a
oIG3004	GCCGGAGGCGCTGCCGGAGCCGGCGCTGCCGTCGCCTCCCAGCTGAGACAG	Cas9-Linker1_rev_pX334a
oIG3005	TGTCTTTTATTTCAGGTCCCGGATCGAATTGCGGCCGCAGGAGGCGCCACCATGTACCCATACGATGTTCCAGATTACGC	Bb-HA(-NLS-Cas9)_forw_pMZ333
oIG3006	TCTGGATCGAAGCTTGGGCTGCAGGTCGACTCTAGATCAGCTGGCCTCCACTTTTCTCTTCTTTTTGGGGCTCTTGTACAGCTCGTCCATGCCGC	mCherry-NLS-Bb_rev_pMZ333
oIG3007	TGTCTTTTATTTCAGGTCCCGGATCGAATTGCGGCCGCAGGAGGCGCCACCATGTACCCATACGATGTTCCAGATTACGCTATGAGAGCTGCCAACCTTTGGCCAAG	Bb-HA-ERT2_forw_pMItom
oIG3008	GGACGCTTCGACCTTGCGCTTTTTCTTCGGCGAGACTGTGGCAGGGAAACCCTCTG	ERT2-NLS(-Cas9)_rev_pMItom
oIG3009	GGGGAGGCAGAGGGTTTCCCTGCCACAGTCTCGCCGAAGAAAAAGCGCAAGGTC	ERT2-NLS(-Cas9)_forw_pX334a
oIG3010	GCTGGCCTCCACTTTTCTCTTCTTTTTGGGGCTCTTGTACAGCTCGTCCATGCCGC	mCherry-NLS3'_rev_pMZ333
oIG3011	AGCCCCAAAAAGAAGAGAAAAGTGGAGGCCAGCATGAGAGCTGCCAACCTTTGGCCAAG	NLS3'-ERT2_forw_pMItom
oIG3012	TCTGGATCGAAGCTTGGGCTGCAGGTCGACTCTAGATCAGACTGTGGCAGGGAAACCCTCTG	ERT2-Bb_rev_pMItom
oIG3013	AAACGATGATGAGGTTCTTGGCGGCTGTCTGTGCGT	crRNA_SEAP(nt136-166)_forw
oIG3014	TAAAACGCACAGACAGCCGCCAAGAACCTCATCATC	crRNA_SEAP(nt136-166)_rev
oIG3015	AAACCTGGCACATGTTTGTCTACATTGTATGTCTGT	crRNA_SEAP(nt293-323)_forw
oIG3016	TAAAACAGACATACAATGTAGACAAACATGTGCCAG	crRNA_SEAP(nt293-323)_rev
oIG4000	GATATGGCGGGGGAACAGGGG	mCherry-fwd_pMZ700
oIG4001	GCCGCTGCCGGCGCCGCTGCCGGCGCTGCCCTTGTACAGCTCGTCCATGCCGC	mCherry-rev_pMZ700/pMZ701
oIG4002	GGCAGCGCCGGCAGCGGCGCCGGCAGCGGCGACAAGAAGTACAGCATCGGCCTG	cas9-fwd_pMZ1025
oIG4003	GTCTGGATCGAAGCTTGGGCTGCAGGTCGACTCTAGATTAGTCGCCTCCCAGCTGAGACAG	cas9-rev_pMZ1025
oIG4004	GTTATTGGGGCTTTCTGTTTCTTGCAAATCC	mCherry-fwd_pMU701
oIG4005	GTCTGGATCGAAGCTTGGGCTGCAGGTCGACTCTAGATTACACCTTTCTCTTCTTCTTGGGGCTGTC	cas9(with NLS)-rev_pMZ1025
oIG4006	TCGAAGCTTGGGCTGCAGGTCGACTCTAGATTACACCTTTCTCTTCTTCTTGGGGCTGTCGCCTCCCAGCTGAGACAG	cas9(with NLS)-rev_pIG4004 and pIG4005
oIG5000	TGCCTGAAATCACTTTTTTTCAGGTTGGACCGGTGCCACCTCGCGAATGTACCCATACGATGTTCCAGATTACG	Backbone-Nru1-HA_for_pX334a
oIG5001	GGAGGCGGTGGCAGTGGTGGCGGAGGCAGTGGATCCATGGCCGACAAGCAGAAGAACGGC	(GGGGS)3_Linker-BamH1-splitC_YFP_for_BBa_K323080
oIG5002	AAGTTAGTAGCTCCGCTTCCGCTGGCCTCCACCTTTCTCTTCTTCTTGGGGCTGTCGACCTTGTACAGCTCGTCCATGCCGAG	P2A(first 20bp)-NLS-Sal1-splitC_YFP_rev_BBa_K323080
oIG5003	GGAGGCGGTGGCAGTGGTGGCGGAGGCAGTGGATCCGTGAGCAAGGGCGAGGAGCTG	(GGGGS)3_Linker-BamH1-splitN_YFP_for_BBa_K323005
oIG5004	AAGTTAGTAGCTCCGCTTCCGCTGGCCTCCACCTTTCTCTTCTTCTTGGGGCTGTCGACGATGTTGTGGCGGATCTTGAAGTTCAC	P2A(first20 bp)-NLS-Sal1-splitN_YFP_rev_BBa_K323005
oIG5007	GGAAGCGGAGCTACTAACTTCAGCCTGCTGAAGCAGGCTGGAGACGTGGAGGAGAACCCTGGACCTATGGTGAGCAAGGGCGAGGAGG	P2A-mCherry_for_pMH206
oIG5008	CAACTAGAAGGCACAGTCGAGGCTGATCAGCGAGCTCTAGTCGCGATTACTTGTACAGCTCGTCCATGCCGC	Backbone(pX334a)-Nru1-mCherry_rev_pMH206
oIG5009	CTAGAGCTCGCTGATCAGCCTCG	forward - pX334a backbone amplification (Part1)
oIG5010	TCAAAAGAATAGCCCGAGATAGGGTTGAG	reverse - pX334a backbone amplification (Part1)
oIG5011	CTTTAATAGTGGACTCTTGTTCCAAACTGGAAC	forward - pX334a backbone amplification (Part2)
oIG5012	GGTGGCACCGGTCCAACCTG	reverse - pX334a backbone amplification (Part2)
oIG5013	ACTGCCTCCGCCACCACTGCCACCGCCTCCGTCGCCTCCCAGCTGAGACAG	(GGGGS)3_Linker-hCas9_rev_pX334a
oIG5014	GGAGGCGGTGGCAGTGGTGGCGGAGGCAGTGGATCCATGGAGAACACTGAAAACTCAGTGGATTC	(GGGGS)3_Linker-BamH1-Caspase3_for_pMK31
oIG5015	AAGTTAGTAGCTCCGCTTCCGCTGGCCTCCACCTTTCTCTTCTTCTTGGGGCTGTCGACATGATGATGATGATGATGGTGATAAAAATAGAGTTC	P2A(first 20bp)-NLS-Sal1-Caspase3_rev_pMK31
oIG5016	CTTAGCGGCAGCTTCCTTAGCGGCAGCTTCGTCGCCTCCCAGCTGAGACAG	(EAAAK)3_Linker-hCas9_rev_pX334a
oIG5017	GAAGCTGCCGCTAAGGAAGCTGCCGCTAAGGGATCCATGGAGAACACTGAAAACTCAGTGGATTCAAAATC	(EAAAK)3_Linker-BamH1-Caspase3_for_pMK31
oIG5018	GGAGGCGGTGGCAGTGGTGGCGGAGGCAGTGGATCCAGGCAAGCAGCAAAGTTGTCGAAGCC	(GGGGS)3_Linker-BamH1-Casp9_for_pKJ42
oIG5019	AAGTTAGTAGCTCCGCTTCCGCTGGCCTCCACCTTTCTCTTCTTCTTGGGGCTGTCGACTGATGTTTTAAAGAAAAGTTTTTTCCGGAGG	P2A(first 20bp)-NLS-sal1-Casp9(long)_rev_pKJ42
oIG5021	GAAGCTGCCGCTAAGGAAGCTGCCGCTAAGGGATCCAGGCAAGCAGCAAAGTTGTCGAAGCC	(EAAAK)2-BamH1-Casp9_for_pKJ42
oIG5022	GTCTATATAAGCAGAGCTCTCTGGCATGAGCCCCAAGAAGAAGAGAAAGGTGGAGGCCAGCATGTCGAATTCTTATGACTCCTCCAGTATC	Backbone(pcDNA_3.1)-NLS-GyrB-Caspase3_for_pMK31
oIG5023	GTCTATATAAGCAGAGCTCTCTGGCATGAGCCCCAAGAAGAAGAGAAAGGTGGAGGCCAGCAGGCAAGCAGCAAAGTTGTCGAAGC	Backbone(pcDNA_3.1)-NLS-Caspase9-GyrB_for_pKJ42
oIG5024	AAGTTAGTAGCTCCGCTTCCGCTGGCCTCCACCTTTCTCTTCTTCTTGGGGCTGCCTTCATAGTGGAAGTGGTCTTCTTTG	P2A(first 20bp)-NLS-Caspase9-GyrB_rev_pKJ42
oIG5025	ACTGCCTCCGCCACCACTGCCACCGCCTCCGCCTTCATAGTGGAAGTGGTCTTCTTTG	(GGGGS)2_Linker-GyrB_rev_pMK31
oIG5026	ATGGCTGGCAACTAGAAGGCACAGTCGAGGTCACTTGTACAGCTCGTCCATGCCGC	Backbone(pcDNA_3.1)-mCherry_rev_pMH206
oIG5027	CCTCGACTGTGCCTTCTAGTTGCC	forward - pcDNA_3.1 backbone amplification (Part1)
oIG5028	CACTTTATGCTTCCGGCTCGTATGTTG	reverse - pcDNA_3.1 backbone amplification (Part1)
oIG5029	CCACACAACATACGAGCCGGAAGC	forward - pcDNA_3.1 backbone amplification (Part2)
oIG5030	GCCAGAGAGCTCTGCTTATATAGACC	reverse - pcDNA_3.1 backbone amplification (Part2)
oIG5031	CAGGTTGGACCGGTGCCACCTCGCGAATGAGCCCCAAGAAGAAGAGAAAGGTGGAGGCCAGCGGATCCAGGCAAGCAGCAAAGTTGTCGAAGC	Backbone(pX334a)-Nru1-NLS-BamH1-Caspase9(long)_for_pKJ42
oIG5032	ACTGCCTCCGCCACCACTGCCACCGCCTCCGTCGACTGATGTTTTAAAGAAAAGTTTTTTCCGGAGG	(GGGGS)2_Linker_Sal1_Caspase9(long)_rev_pKJ42
oIG5033
oIG5034	GGAGGCGGTGGCAGTGGTGGCGGAGGCAGTGACAAGAAGTACAGCATCGGCCTGG	(GGGGS)2_Linker-hCas9-NLS_for_pX334a
oIG5035	CTTCAGCAGGCTGAAGTTAGTAGCTCCGCTTCCAGCGTAATCTGGAACATCGTATGGGTACATGCTGGCCTCCACCTTTCTCTTCTTC	P2A(first 33bp)-HA-hCas9-NLS_rev_pX334a
oIG6000	TCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGCAGCTAGCTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTC	pCMVfor-psB1C3
oIG6001	CGAGCGGTCCGCGGAACCAGCGCTACCGGACTGCCATTGCTCCGGCTGATCTGACGGTTCACTAAACCAGCTC	pCMVrev-eGFP
oIG6002	AGCCGGAGCAATGGCAGTCCGGTAGCGCTGGTTCCGCGGACCGCTCGGCCACCATGGTGAGCAAGGGC	eGFPfor-CMV
oIG6003	GGACGCTTCGACCTTGCGCTTTTTCTTCGGCGAGGGATCCGACCCGTCGACGCCCTTGTACAGCTCGTCCATGC	eGFPrev-Sal1-Bamh1-NLS
oIG6004	TCGCCGAAGAAAAAGCGCAAGGTCGAAGCGTCCTAAAGATCTATAATCAGCCATACCACATTTGTAGAGGTTTTAC	terminatorfor-NLS
oIG6005	GCCGGACTGCAGCGGCCGCTACTAGTAAGGTACCGTAAGCTTTAGCTCTGAATGCATGCGTATTGCTAATGGGTCTAATGCAGTTAAGATACATTGATGAGTTTGGACAAACCAC	terminatorrev-Nru1-Hind3-pSB1C3-Spe1-Not1-Pst1
oIG6006	AAACCCCTCGCCCTCGCCGGACACGCTGAACTTGGT	eGFPfw
oIG6007	TAAAACCAAGTTCAGCGTGTCCGGCGAGGGCGAGGG	eGFPrv
oIG6008	AAACGGCGGGCCATTTACCGTAAGTTATGTAACGGT	CMVfw
oIG6009	TAAAACCGTTACATAACTTACGGTAAATGGCCCGCC	CMVrv
oIG6010	AAACTTATGTTTCAGGTTCAGGGGGAGGTGTGGGGT	sv40 terminatorfw
oIG6011	TAAAACCCCACACCTCCCCCTGAACCTGAAACATAA	sv 40 terminatorrv
oIG6012	GGACGCTTCGACCTTGCGCTTTTTCTTCGGCGAGGGATCCGACCCGTCGACGCCTTACTTGTACAGCTCGTCCATGCCG	eCFPrv
oIG605neu	GTTCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTAAGGTACCGTAAGCTTTAGCTCTGAATGCATGCGTATTGCTAATGGGTCTAATGCAGTTAAGATACATTGATGAGTTTGGACAAACCAC	rv primer für den terminator mit mehr basen overlapp
oIG6015	ATGTACACTAAGTCTAGCTAGACTAGCTGGTACCGACTAGTAGCGGCCGCTGCAGTC
oIG6016	GCTATAGCTAAGCGCGTCGAGTCGAGCGAAGCTTATCTAGAAGCGGCCGCGAATTCCAG
oIG6017	GAGTGCCACCTGACGTCTAAGAAAC	primer für pSB1C3 fw
oIG6018	CGCCTTTGAGTGAGCTGATACCGC	primer für pSB1C3 rv
oig6020	TCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGCAGCTAGCCGATGTACGGGCCAGATATACGCG	CMV fw
oig6021	CGAGCGGTCCGCGGAACCAGCGCTACCGGACTGCCATTGCTCCGGCTAATTTCGATAAGCCAGTAAGCAGTGGGTTC	CMV rv
oig6022	TCGCCGAAGAAAAAGCGCAAGGTCGAAGCGTCCTAAAGATCTCGACTGTGCCTTCTAGTTGCCAGC	bgh terminator fw
oig6023	GCCGGACTGCAGCGGCCGCTACTAGTAAGGTACCGTAAGCTTTAGCTCTGAATGCATGCGTATTGCTAATGGGTCTAATGCACCATAGAGCCCACCGCATCCC	bgh terminator rv
oig6024	AGCCGGAGCAATGGCAGTCCGGTAGCGCTGGTTCCGCGGACCGCTCGGCCACCATGGCCACAACCATGGTGAGCAAGG	eCFP fw
oig6025	GGACGCTTCGACCTTGCGCTTTTTCTTCGGCGAGGGATCCGACCCGTCGACGCCCTTGTACAGCTCGTCCATGCCGAG	eCFP rv
oig6030	AAACGTTCAAGTCGCACTCGCCGCTCCCGCTCCCGT	AvGFP fw beginning
oig6031	TAAAACGGGAGCGGGAGCGGCGAGTGCGACTTGAAC	AvGFP rv beginning
oig6032	AAACGTAACTGCGTTTACCCGCCATCCGCACATGGT	target CMV promoter before tata fw
oig6033	TAAAACCATGTGCGGATGGCGGGTAAACGCAGTTAC	target CMV promoter before tata rv
oig6034	AAACAGTCTAGTCGGCCTCGTTACCGTCAGGCCAGT	target CMV behind tata fw
oig6035	TAAAACTGGCCTGACGGTAACGAGGCCGACTAGACT	target CMV behind tata rv
oig6036	AAACAGATCAACGGTCGGTAGACAACAAACGGGGGT	target bgh terminator beginning fw
oig6037	TAAAACCCCCGTTTGTTGTCTACCGACCGTTGATCT	target bgh terminator beginning rv
oig6038	AAACGTTCAAGTCGCACAGGCCGCTCCCGCTCCCGT	target eCFP fw
oig6039	TAAAACGGGAGCGGGAGCGGCCTGTGCGACTTGAAC	target eCFP rv
oIG8002	GCCGGCGGAGCCTGCTGATCCGGGGCTGTCGCCTCCCAGC	Cas9_GSAGSAG_rev
oIG8005	GGACGCTTCGACCTTGCGCTTTTTC	NLS1_pX334_rev
oIG8006	GGATCAGCAGGCTCCGCCGGCGACAAGAAGTACAGCATCGGCCTG	Cas9_GSAGSAG_fw
oIG8007	GGTGCTGGTATGACAAG	G9a_seq_fw_pGEX2T
oIG8008	GATGTTGCCATAGTAACGG	G9a_seq_rev_pGEX2T
oIG8009	GACAGCCCCGGATCAGCAGGCTCCGCCGGCGAGAATGTACCCATCCCCTGTGTC	G9a(mm)_C-term_GSAGSAG_fw
oIG8010	GGATCCTCACACCTTCCGCTTTTTCTTGGGGGTGTTGATGGGGGGCAGGGA	G9a(mm)_C-term_NLS_bgh_rev
oIG8011	AAGCGCAAGGTCGAAGCGTCCGAGAATGTACCCATCCCCTGTGTC	G9a(mm)_N-term_NLS_fw
oIG8012	GCCGGCGGAGCCTGCTGATCCGGTGTTGATGGGGGGCAGGGA	G9a(mm)_N-term_GSAGSAG_rev
oIG8013	GCCCGTTACTATGGCAACATCAGCCGATTCATTAACAAGCTGTGTGACCCCAACATCATCCCTGTCCGGGTTTTCATGCTG	dG9a(mm)_H->K_fw
oIG8014	CAGCATGAAAACCCGGACAGGGATGATGTTGGGGTCACACAGCTTGTTAATGAATCGGCTGATGTTGCCATAGTAACGGGC	dG9a(mm)_H->K_rev
oIG8015	GAATTCGCGGCCGCTTCTAGATGGCCGGCGAGAATGTACCCATCCCCTGTGTC	_G9a_fw_std
oIG8016	CTGCAGCGGCCGCTACTAGTATTAACCGGTGGTGTTGATGGGGGGCAGGGA	G9a_rev_std
oIG8017	GCAGTCATCCACACACGTGCAGTGCTGGAGATGGGTGATGTTGCGGTCGA	G9a_1stsite_std_rev
oIG8018	CAGCACTGCACGTGTGTGGATG	G9a_1stsite_std_fw
oIG8019	GCCCATCTTGGCAGTCCGGTAGAGCTGGAGCCGTACCTTGATGCCGCTCT	G9a_2ndsite_std_rev
oIG8020	CAGCTCTACCGGACTGCCAAGA	G9a_2ndsite_std_fw
oIG8021	CCCAAGAAAAAGCGGAAGGTGTGATC	phyB hole vector for
oIG8022	GCGGCTGTACGCGGAACCAG	phyB hole vector rev
oIG8023	GCTGGTAGTGCTGGTTCCGCGTACAGCCGCGAGAATGTACCCATCCCCTGTGTC	phyB(overhang)_g9a for
oIG8024	CTCTAGATCACACCTTCCGCTTTTTCTTGGGGGTGTTGATGGGGGGCAGGGA	phyB(overhang)_g9a rev
oIG9100	TCATATCCGCGGCATGTACCCATACGATGTTCCAGATTACG	cas9 fw incl. HA tag and NLS
oIG9191	GATATTGGTACCCATGCCTGCTATTCTCTTCCCAATCCTC	cas9 rv incl. Bgh terminator
oIG4100F	GCTAGCTAGTTATTAATAGTAATCAATTACGGG	wird nachgetragen
oIG4100R	GAATTCGATCTGACGGTTCACTAAAC
oIG4101F	ATGTACCCATACGATGTTCCAG
oIG4101R	GGGGCTGTCGCCTCCCAG
oIG4102F	GACACGGATCGACCTGTCTCAGCTGGGAGGCGACAGCCCCCCGAAGAAAAAGCGCAAGGTCGCGCTCGATAGTGCTGGTAG
oIG4102R	TCTTCGGCGAAGCGTAATCTGGAACATCGTATGGGTACATGGTGGGCGGCCGCAATTC
oIG4103F	ATGGATGCTAAGTCACTAACTGCC
oIG4103R	TCACACCTTCCGCTTTTTCT
oIG4104F	AAGGAATGATCTCTGGCCCAAGAAAAAGCGGAAGGTGTGATCTAGAGTCGACCTGCAGCC
oIG4104R	CCAGTGTCCGGGACCAGGCAGTTAGTGACTTAGCATCCATGCGGCTGTACGCGGAACCAG
oIG4105F	GACACGGATCGACCTGTCTCAGCTGGGAGGCGACAGCCCCCCGAAGAAAAAGCGCAAGGTCGGTACCGGCGCCGGCG
oIG4105R	TCTTCGGCGAAGCGTAATCTGGAACATCGTATGGGTACATGGTGGGAATTCGCGGCC
oIG4106F	AAGGAATGATCTCTGGCCCAAGAAAAAGCGGAAGGTGTGAGGGGCGCGAGGATCCTCTAG
oIG4106R	CCAGTGTCCGGGACCAGGCAGTTAGTGACTTAGCATCCATCGCGCCGGCGCCCGCAG
oIG4107F	GTCTCAGCTGGGAGGCGACAGCCCCCCGAAGAAAAAGCGCAAGGTCGGTAGTGCTGGTAGTGCTGGTAGTGCTGGTATGAAGATGGACAAAAAGACTATAGTT
oIG4107R	TGCTGCTCCGATCATGATCTG
oIG4108F	TTCAAGAACCCGTGAAGCACAGATCATGATCGGAGCAGCATGATCTAGAGTCGACCTGCAGC
oIG4108R	TCTTCGGCGAAGCGTAATCTGGAACATCGTATGGGTACATGGTGGGCGGCCGCAATTC
oIG4109F	GATAGTGCTGGTAGTGCTGGT
oIG4109R	GGTGGGCGGCCGCAATTC
oIG4110F	CTTTTATTTCAGGTCCCGGATCGAATTGCGGCCGCCCACCATGAATGGAGCTATAGGAGGTGAC
oIG4110R	TGTACGCGGAACCAGCACTACCAGCACTACCAGCACTATCATGAATATAATCCGTTTTCTCCAATTCCT
oIG4111F	AAGGAATGATCTCTGGCCCAAGAAAAAGCGGAAGGTGTGATCTAGAGTCGACCTGCAGCC
oIG4111R	GGTGGGCGGCCGCAATTC
oIG4112R	AGGCAGTTAGTGACTTAGCATCCATGCGGCTGTACGCGGAACCAGCACTACCAGCACTACCAGCACTATCATGAATATAATCCGTTTTCTCCAATTCCT
crRNA tetO 1 F	TAAAACATCAGTGATAGAGAAAAGTGAAAGTCGAGT
crRNA tetO 1 R	AAACACTCGACTTTCACTTTTCTCTATCACTGATGT
crRNA tetO 2 F	TAAAACCTCCCTATCAGTGATAGAGAAAAGTGAAAG
crRNA tetO 2 R	AAACCTTTCACTTTTCTCTATCACTGATAGGGAGGT