Chemical reasoning

There are 6 enzyms involved in the natural process of the enterobactin production:

EntA :
EntB :
EntC :
EntD :
EntE :
EntF :

The production of each one of those enzymes can thus become a rate-limiting-step, when it comes to mass enterobactin production.

For now, we consider each one of these steps as a simple chemical reaction:

We are using the enzymatic kinetic model of Michaelis-Menten, which divides each reaction into two consecutives steps:

The speed of the reaction is calculated as below:

The steady state approximation gives us:

And thus,

where and are classic kinetic parameters.

Important result:

For simple enzymatic reactions (one reagent, one product and one enzyme) with the steady state approximation, we can directly determine the speed:

Team:Evry/ChemicalTools

From 2013.igem.org

Chemical reasoning

Important result: