Electro-Elution

1. Cut the gel strip under the UV plate, then cut the gel in little pieces

2. Fill the vat with TAE 0.5X buffer

3. Close one of the wells of the saline bridge with a semi-permeable membrane, put the DNA in the large well

4. Apply a 400V current after closing the vat, during 30min

5. Reverse the polarity during 20s, pipet the solution from the small well and put it in a clean 1.5 ml tube

6. Add 200µL of water and 600µL of phenol/chloroform/isoamyl alcohol 25/24/1

7. Vortex during 1 min

8. Pipet the aqueous phase

9. Precipitation the DNA with ethanol (see protocol)