Team:Evry/Sensor

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Iron coli project

Sensor

We constructed iron sensors by combining 3 genetic parts: an E. coli promoter with a Fur binding site, the sfGFP fluorescent reporter, and a transcriptional terminator. These iron sensing elements were cloned in Biobrick compatible format as shown below.

NAME FIGURE DESCRIPTION

E. coli promoter with Fur binding site

iron-Fur complex binds promoter to repress expression

sfGFP

Fluorescent reporter gene

Terminator

terminator to stop transcription

Plasmid

Biobrick-compatible plasmid backbone

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