Team:TU-Munich/Results/Overview

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Results Overview

Until the regional Jamboree in Lyon we were able to create 72 BioBricks and devices, have successfully transformed and selected xx transgenic GM-Moss. Different effectors were characterized as recombinant proteins, which were produced in E. coli but also as transformation product in our moss Physcomitrella patens. Beside these activities we contributed to the progress of the iGEM community by writing a software tool for the annotation of BioBricks. We also wrote tutorials to conserve the knowledge we have gathered during this summer for subsequent generations of iGEM students. Furthermore we developed a concept based on swimming triangular pods. This concept shows how our PhyscoFilter could be implemented in the environment. Furthermore we analyzed the economic potential of this innovative technology in our Entrepreneurship section.

BioBricks

During the summer the team has created 72 BioBricks which can mainly be sub-grouped into Physcomitrella patens as a chassis, Phytoremediation BioBricks and light triggered kill-switch (Read more).

Effectors

We chose six different effector proteins which we have produced recombinantly in Escherichia coli to characterize them on a molecular level concerning stability and activity (Read more).

WT-Moss

Due to no research on Physcomitrella patens at our university, we had to make some general experiments with this moss concerning tolerance of toxin, growing properties on different surfaces and the determination of growth rates (Read more).

GM-Moss

In our project we created 25 different strains of stabily transformated moss, which was done 350 km away in Freiburg. We then selected and regenerated transgenic moss over 4 weeks and tested several of them in 96-well plates (Read more).

Software

Protein coding BioBricks are an important point in the Parts Registry. As they are standardized by definition, our AutoAnnotator translates the sequence, computes different valuable parameters, does alignments and presents all these information as a formated table as output (Read more).

Tutorials

During the summer we learned a lot about iGEM and found solutions for problems which appear in many iGEM projects. So we want to share this knowledge for the benefit of the community. Therefore we created our tutorial section (Read more).

Implementation

As soon as transgenic moss with a phytoremediation activity is available, it becomes necessary to develop a solution of how to implement a SynBio waterfilter into the environment. For this purpose we present a concept of swimming triangular pods (Read more).

Entrepreneurship

Science is an extremely important point for progress, but in the end it is essential to develop a business model oppon which the technology can be brought to market (Read more).

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