Team:TU-Munich/Project/Overview

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Project Overview

In the 2013 competition the TU Munich iGEM team has developed a transgenic moss filter which is capable to reduct the contamination of aquatic ecosystems with xenobiotics. The topic of remediation using transgenic organisms is present in every year of iGEM and we want to take this idea to the next level. For this reason we decided to use a photoautotrophic chassis which we enabled for the iGEM competition. The following section gives an overview over the theoretical background of our project.

Phytoremediation

Phytoremediation describes the treatment of environmental problems through the use of plants. Here we identified problematic substances and described basic principles (Read more).

Physcomitrella

Next, we introduce Physcomitrella patens as a nearly new chassis to iGEM. We describe advantages, possiblilities and our expression strategy (Read more).

Localization

As the principle of action for the effectors, responsible for the Phytoremediation, are very different, it is necessary to have a protein expression system which is able to accomplish cytosolic, secreted and receptor bound localization of proteins (Read more).

BioDegradation

BioDegradation describes the application of enzymatic catalysis for the degradation of problematic xenobiotics using the enzymes erythromycin esterase, catechol dioxigenase and laccase (Read more).

BioAccumulation

(Read more).

Kill-Switch

In order to restrict the uncontrollable growth of transgenic moss in the environment, we developed a Kill-Switch which is triggered by sunlight. The GM-Moss can only be grown where red-light is filtered out of the electromagnetic spectrum (Read more).

Safety

Our safety page describes our team discussions concerning safety issues, especially a safety evaluation of our BioBricks and Physcomitralla patens, including all constructed GM-Moss plants(Read more).

Implementation

The creation of new environmental solutions doesn't stop at the development of transgenic moss. We tried to find out how a implementation of a large-scale biofilter could look like. Taking account of recent issues such as limited space and missing vegetation in urban areas. (Read more).

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