"
Team:Evry/Chelator
From 2013.igem.org
Iron Chelator
Assembly of enterobactin chelator genes entA-entF into a single operon
Fig 1.a
Fig 1.b
| NAME | FIGURE | Description |
|---|---|---|
|
Lac promoter |
|
Lac Promoter |
|
RBS + EntA |
|
First gene required for enterobactin sythesis |
|
RBS + EntB |
|
Second gene required for enterobactin sythesis |
|
RBS + EntC |
|
Third gene required for enterobactin sythesis |
|
RBS + EntD |
|
Fourth gene required for enterobactin sythesis |
|
RBS + EntE |
|
Fifth gene required for enterobactin sythesis |
|
RBS + EntF |
|
Sixth gene required for enterobactin synthesis |
|
Terminator |
|
Transcription Stop signal |
|
Plasmid |
|
Backbone with ampicillin resistance |
Table 1. Genetic elements used to produce the enterobactin siderophore.
Future caracterisation of the construction
The first
