"
Team:Evry/Chelator
From 2013.igem.org
Iron Chelator
Here we present Fig 1.a and 1.b our constructions Assembly of enterobactin chelator genes entA-entF into a single operon
Fig 1.a First construction containing the Lac I regulated enterobactin synthesis genes Ent A, Ent D and Ent F. Genes fusions were made with flanking restriction sites that are compatible with Biobrick-based cloning.
Fig 1.b Fig 1.a Second construction containing the Lac I regulated enterobactin synthesis genes Ent B, Ent C and Ent E. Genes fusions were made with flanking restriction sites that are compatible with Biobrick-based cloning.
| NAME | FIGURE | Description |
|---|---|---|
|
Lac promoter |
|
Lac Promoter |
|
RBS + EntA |
|
First gene required for enterobactin sythesis |
|
RBS + EntB |
|
Second gene required for enterobactin sythesis |
|
RBS + EntC |
|
Third gene required for enterobactin sythesis |
|
RBS + EntD |
|
Fourth gene required for enterobactin sythesis |
|
RBS + EntE |
|
Fifth gene required for enterobactin sythesis |
|
RBS + EntF |
|
Sixth gene required for enterobactin synthesis |
|
Terminator |
|
Transcription Stop signal |
|
Plasmid |
|
Backbone with ampicillin resistance |
Table 1. Genetic elements used to produce the enterobactin siderophore.
Future caracterisation of the construction
The first
