Iron Chelator

First strategy for enterobactin biosynthesis

Here we present Fig 1 and 2 our constructions which contain each three Lac I regulated enterobactin synthesis genes. Escherichia coli naturally have those genes into a single operon but due to their important lenghts, we decided to divide them into two indivudual constructions in order to make the cloning easier.

Fig 1 First construction containing the Lac I regulated enterobactin synthesis genes Ent A, Ent D and Ent F. Genes fusions were made with flanking restriction sites that are compatible with Biobrick-based cloning.

Fig 2 Second construction containing the Lac I regulated enterobactin synthesis genes Ent B, Ent C and Ent E. Genes fusions were made with flanking restriction sites that are compatible with Biobrick-based cloning.

NAME	FIGURE	Description
Lac promoter		Lac Promoter
RBS + EntA		First gene required for enterobactin sythesis
RBS + EntB		Second gene required for enterobactin sythesis
RBS + EntC		Third gene required for enterobactin sythesis
RBS + EntD		Fourth gene required for enterobactin sythesis
RBS + EntE		Fifth gene required for enterobactin sythesis
RBS + EntF		Sixth gene required for enterobactin synthesis
Terminator		Transcription Stop signal
Plasmid		Backbone with ampicillin resistance