Labwork Description

Within the scope of the 2013 iGEM competition our goal was to create tumor targeting bacteria device which, upon the sensing of a tumor, would produce proteins that could be used for the imaging thereof. To achieve this, it was necessary to design and develop a system that targeted and reacted to tumors, and was able to produce the proteins that enable the tumor imaging. In order to develop such a system, we first defined the DNA CONSTRUCTS that constitute the basis of all our lab work. To ensure that the best possible protein was used for the tumor imaging it was decided to design and produce multiple different proteins. Once we generated our CEST contrast agent device, we TESTED THE CEST CONTRAST AGENT obtained by producing different proteins and its imaging qualities. The latter was done with a MRI SCANNER. We also evaluated the ability of our device to generate the contrast agent under hypoxic conditions by performing an ANAEROBIC TESTING of the FNR Promoter. The complete procedures and protocols that we performed within our lab work can be found on the LAB JOURNAL.