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(click here to learn more about our circuit)
Originally, we use CI promoter, which will be inhibited by CI protein, to repress kill protein production when there’s no Nosema invasion. However the result shows that kill protein's full expression is too low to kill Nosema even though CI is absent.
Therefore, we replace CI promoter with pLux/CI hybrid promoter. Besides, we also add LuxR, LuxI genes behind to build a positive-feedback mechanism for kill protein to reach its effective level and kill Nosema in time.
