Team:Paris Bettencourt/Notebook

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PROJECT
- OVERVIEW
- DETECT
- TARGET
- INFILTRATE
- SABOTAGE
ACHIEVEMENTS
HUMAN PRACTICE
SAFETY
COLLABORATION
- COLLABORATION
- SENSIGEM
NOTEBOOK
TEAM

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Notebook

Contents

1 Week 01: 1^st - 7^th July
2 Week 02: 8^th - 14^th July
3 Week 03: 15^th - 21^th July
4 Week 04: 22^th - 28^th July
5 Week 05: 29^th July - 4^th August
6 Week 06: 5^th - 11^th August
7 Week 07: 12^th - 18^th August
8 Week 08: 19^th - 25^th August
9 Week 09: 26^th August - 1^st September
10 Week 10: 2^nd - 8^th September
11 Week 11: 9^th - 15^th September
12 Week 12: 16^th - 22^th September
13 Week 13: 23^th - 29^th September
14 Week 14: 30^th September - 6^th October
15 Week 15: 7^th - 13^th October
16 Week 16: 14^th - 20^th October
17 Week 17: 21^th - 27^th October
18 Week 18: 28^th October - 3^th November

Week 01: 1^st - 7^th July

Monday 01^st July

Drug Screening

Media was made, Strains sD001-sD004 were received, inoculated, and put into stock and catalog.

4 strains were recieved from Jake:

E. coli: BL21 (DE3) ko20 ΔcysI, Δfpr, ΔydbK
E. coli: NEBTurbo zmSIR Chloramphenicol
E. coli: NEBTurbo zmFNR Spectinomycin
E. coli: NEBTurbo soFD, zmSIR Chloramphenicol

Media and Glycerol stock were prepared:

Media Preparation

3 500ml bottles of LB broth and LB agar were prepared by standard methods.
12.5g/500ml powder/water for broth and 20g/500ml powder/water for agar.
Bottles were autoclaved. Additionally 1 flask of 500ml of LB broth was made in the same fashion.

Glycerol Stocks

Single colonies from agar plates were picked and used to innoculate 5ml LB broth overnight.
750ml of overnight culture was added to 250ml of 60% glycerol in a cryotube.
Two sets of Glycerol stocks were used for each of sD001, sD002, sD003, sD004; one set was frozen at -20ºC and the other set was frozen at -80ºC.

Phage Sensor

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Trojan Horse

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TB-ception

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Tuesday 02^st July

Drug Screening

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TB-ception

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Wednesday 03^st July

Drug Screening

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TB-ception

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Thursday 04^st July

Drug Screening

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TB-ception

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Friday 04^st July

Drug Screening

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Phage Sensor

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TB-ception

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Week 02: 8^th - 14^th July

Monday 8^th July

Drug Screening

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TB-ception

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Tuesday 9^th July

Drug Screening

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Wednesday 10^th July

Drug Screening

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Thursday 11^th July

Drug Screening

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Friday 12^th July

Drug Screening

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Week 03: 15^th - 21^th July

Monday 15^th July

Drug Screening

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Tuesday 16^th July

Drug Screening

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Wednesday 17^th July

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Thursday 18^th July

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Friday 19^th July

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Week 04: 22^th - 28^th July

Monday 22^th July

Drug Screening

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Tuesday 23^th July

Drug Screening

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Wednesday 24^th July

Drug Screening

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Thursday 25^th July

Drug Screening

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Friday 26^th July

Drug Screening

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Phage Sensor

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TB-ception

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Week 05: 29^th July - 4^th August

Week 06: 5^th - 11^th August

Week 07: 12^th - 18^th August

Week 08: 19^th - 25^th August

Week 09: 26^th August - 1^st September

Week 10: 2^nd - 8^th September

Week 11: 9^th - 15^th September

Week 12: 16^th - 22^th September

Week 13: 23^th - 29^th September

Week 14: 30^th September - 6^th October

Week 15: 7^th - 13^th October

Week 16: 14^th - 20^th October

Week 17: 21^th - 27^th October

Week 18: 28^th October - 3^th November

Centre for Research and Interdisciplinarity (CRI)
Faculty of Medicine Cochin Port-Royal, South wing, 2nd floor
Paris Descartes University
24, rue du Faubourg Saint Jacques
75014 Paris, France

+33 1 44 41 25 22/25

team2013@igem-paris.org

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