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Team:Evry/Notebook/w1
From 2013.igem.org
Week 1: 17th June - 23rd June
Tuesday, 19th June
As a start for the labwork, we first decided to make competent cells. As a consequence, we grew from glycerol samples three E. coli strains in 2 mL of LB medium.The chemically competent strains chosen are as follows:
BL21
DH5α
TOP10
DH5α
TOP10
Thursday, 20th June
Friday, 21st June
The tree following E. coli strains have been prepared to be chemically competent: BL21, DH5α and TOP10.
To check the quality of our work as well as their competence, each strain has been plated on LB medium with ampicillin, kanamycin or chloramphenicol. We also transformed our bacteria with a random plasmid and plated them on LB medium with ampicillin only. We incubated our petri dishes for the week end at 30°c
