"
Team:BYU Provo/Notebook/SmallPhage/Summerexp/Period2/Dailylog
From 2013.igem.org
| Small Phage July - August Notebook: July 16 - July 31 Daily Log
| ||
|
|
7/16/13 - Started approximately 60mL of BL21 liquid culture overnight.
7/17/13 - The titer from 7.15 was too low to start an actual round of mutagenesis. One more round of propagation was performed for 7.13 Phage Propagation Experiment - Do we want to talk about this round of mutagenesis? phage stock 80e6. 1.5mL of E coli, 90uL of phage.
7/19/13 - Performed spot test for 7.17 Mutagen Concentration Test - Fifth Protocol - Performed titer to determine phage concentration 2 for 7.13 Phage Propagation Experiment
7/22/13 - A lot of ousekeeping:
7/26/13 - Designed protocols for 7.29 Mutagen Concentration Test - Sixth Protocol and decided to conduct an experiment to verify bacteria growth curve (specifics see 7.27 Bacteria Growth Curve After Dilution) - Started 3mL of BL21 liquid culture overnight.
7/27/13 - Conducted 7.27 Bacteria Growth Curve After Dilution
7/28/13 - Started 15mL of BL21 liquid culture overnight. - Started phage liquid culture to test for superinfection.
7/29/13 - Performed part 1 of 7.29 Mutagen Concentration Test - Sixth Protocol. - Did a spot test to determine the concentration phage in yesterday's liquid culture (We couldn't test for superinfection because all the bacteria were lysed.)
7/12/13 - Performed spot test to confirm the titer result from July 10th. Detail procedure listed in 7.8 Mutagen Concentration Test - Fourth Protocol. - Started approximately 6 mL of E coli liquid culture overnight.
7/13/13 - Set up the phage liquid culture for propagation in 7.13 Phage Propagation Experiment.
7/14/13 - Started approximately 30mL of E coli BL21 liquid culture overnight.
| |
