Team:Paris Saclay/Notebook/August/8

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Contents

1 Notebook : August 8
- 1.1 Lab work
  - 1.1.1 Aerobic/Anaerobic regulation system
    - 1.1.1.1 Preparation of the PSB3K3 backbone plasmid
      - 1.1.1.1.1 1 - Electrophoresis of BBa_J004450 digested by EcoRI/Pst1 to check if the digestion was right
      - 1.1.1.1.2 2 - Electrophoresis of BBa_J004450 digested by EcoRI/Pst1 to purify the plasmid

Notebook : August 8

Lab work

Aerobic/Anaerobic regulation system

Preparation of the PSB3K3 backbone plasmid

1 - Electrophoresis of BBa_J004450 digested by EcoRI/Pst1 to check if the digestion was right

Damir, Nadia

IMAGE

Well 1 : 6µL DNA Ladder
Well 2 : 5µL of BBa_J004450 digested by EcoRI/Pst1
Gel : 0.8%

Expected sizes :

PSB3K3 : 2750kb
GFP : 1069kb

We obtained fragments of the right size.

2 - Electrophoresis of BBa_J004450 digested by EcoRI/Pst1 to purify the plasmid

Anaïs

IMAGE

Well 1 : 6µL DNA Ladder
Well 2 : 5µL of BBa_J004450 digested by EcoRI/Pst1
Gel : 0.8%

Expected sizes :

PSB3K3 : 2750kb
GFP : 1069kb

We obtained fragments of the right size.

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