"
Team:Paris Saclay/Notebook/August/8
From 2013.igem.org
Contents |
Notebook : August 8
Lab work
Aerobic/Anaerobic regulation system
Preparation of the PSB3K3 backbone plasmid
1 - Electrophoresis of BBa_J004450 digested by EcoRI/Pst1 to check if the digestion was right
Damir, Nadia
| IMAGE |
|
Expected sizes :
- PSB3K3 : 2750kb
- GFP : 1069kb
We obtained fragments of the right size. Now we can purify the PSB3K3 plasmid.
2 - Gel purification of the PSB3K3 plasmid from BBa_J004450 digested by EcoRI/Pst1
2.1 - Migration of the remaining 45µL of BBa_J004450 digested by EcoRI/Pst1
Anaïs
| IMAGE |
|
Now we can do a gel purification of the highest band which contains the PSB3K3 plasmid.
2.2 - Migration of the remaining 45µL of BBa_J004450 digested by EcoRI/Pst1
Nadia
