Notebook : August 8

Lab work

Aerobic/Anaerobic regulation system

Preparation of the PSB3K3 backbone plasmid

1 - Electrophoresis of BBa_J004450 digested by EcoRI/Pst1 to check if the digestion was right

Damir, Nadia

Expected sizes :

• PSB3K3 : 2750kb
• GFP : 1069kb

We obtained fragments of the right size. Now we can purify the PSB3K3 plasmid.

2 - Gel purification of the PSB3K3 plasmid from BBa_J004450 digested by EcoRI/Pst1

2.1 - Migration of the remaining 45µL of BBa_J004450 digested by EcoRI/Pst1

Anaïs

Now we can do a gel purification of the highest band which contains the PSB3K3 plasmid.

2.2 - Electroelution of the highest band to extract the PSB3K3 plasmid from the gel

Nadia

Protocol : Electroelution

We let the plasmid precipitate during the night.