Team:Paris Saclay/Notebook/August/6

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Notebook : August 6

Lab work

A - Aerobic/Anaerobic regulation system

Obtaining activator FNR promoter (narK, nirB, narG)

1 - Gel electrophoresis of the colony PCR products ( made the 08/05/2013)on 25 colonies

XiaoJing, Damir, Anaïs

  • NarK promoter in psB1C3
IMAGE
  • Wells 1 to 25 : 10µl sample
  • Well between 12 and 13 : 6µl gene ruler
  • Gel : 1.5%

Expected size : 500 bp

We obtained fragments of the right size.Our backbone contains the Biobrick.

  • NirB promoter in psB1C3
IMAGE
  • Before well 1  : 6µl gene ruler
  • Wells 1 to 6 : 10µl sample
  • Between 6 and 7: 6µl gene ruler
  • Wells 7 to 12 : 10µl sample
  • After well 12 :6µl gene ruler
  • Before well 13  : 6µl gene ruler
  • Wells 13 to 18 : 10µl sample
  • Between 18 and 19: 6µl gene ruler
  • Wells 19 to 25 : 10µl sample
  • After well 25 :6µl gene ruler
  • Gel : 1.5%

Expected size : 500 bp

We obtained fragments of the right size.Our backbone contains the Biobrick.

  • NarG promoter in psB1C3
IMAGE
  • Before well 1  : 6µl gene ruler
  • Wells 1 to 6 : 10µl sample
  • Between 6 and 7: 6µl gene ruler
  • Wells 7 to 12 : 10µl sample
  • After well 12 :6µl gene ruler
  • Before well 13  : 6µl gene ruler
  • Wells 13 to 18 : 10µl sample
  • Between 18 and 19: 6µl gene ruler
  • Wells 19 to 25 : 10µl sample
  • After well 25 :6µl gene ruler
  • Gel : 1.5%

Expected size : 500 bp

We obtained fragments of the right size.Our backbone contains the Biobrick.

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