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KillSwitch
From 2013.igem.org
Kill Switch
The kill switch design is based on the expression of holin and antiholin. Holin is a protein that forms pores in cell membranes. Anti-holin binds to holin and inhibits it's action. Once pores are formed by holin, lysozyme can access the periplasmic space and degrade the cell wall, causing cell lysis.
Kill switch constitutes a crucial part of our final system. It is activated after the production and release of the antimicrobial peptide. In that way, E.coli is killed and the safety of the system is ensured.
