Team:Leeds/Results

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Revision as of 14:44, 21 August 2013 by Emilyjtay (Talk | contribs)

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Experimental Results
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Results from the lab so far. Please note, many of the images have been uploaded at high resolution, and are best viewed from their file page. Access this by clicking the image. We may later introduce a gallery of the images to maximise the screen usage, but this is dependent upon Coffee-Induced coding by the Code Monkey.

Contents

Bacterial growth curves

We are monitoring the growth of our bacteria containing our devices to see if the genes we inserted have any effect on bacterial growth.

Control Bacterial Growth Curve

Control growth-curve for Competent Cells, click for full image

In this experiment we used our chosen expression cells and monitored their growth on SB media both in the presence of chloramphenicol and without chloramphenicol. As the bacteria do not contain our plasmid that codes for chloramphenicol resistance, we didn't expect them to grow when it was present. The graph produced from the absorbance measurements taken over time, shows the growth of the bacteria. It has a clear lag and log phase when the bacteria are grown in SB media only. But as expected, the graph shows that the bacteria do not grow in the presence of chloramphenicol. This graph will be used as a control and other growth curves of bacteria containing our device will be compared to it in order to determine whether the genes we inserted cause an effect on cell growth.

Characterisation of Device 1


Growth Curve


Growth curve for Device 1


Restriction Digests


File:Leeds BS1Restrictiondigests.png

Plate Imaging



Membrane Stress - Hydrophobic Stress


Membrane Stress - Changes in pH


Membrane Stress- Atomic Force Microscopy


Characterisation of Device 2

Growth Curve

Growth curve for Device 2


Restriction Digests


Plate Imaging


Binding assay-washing beads


Binding assay-Confocal Microscopy


Characterisation of Device 3


Growth Curve

Growth curve for Device 3


Restriction Digests


Plate Imaging


Binding Assay-Beads cause fluorescence?


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Geneious, our fine sponsors and suppliers of software Bioline, our fine sponsors and suppliers of equipment Qiagen, our fine sponsors and suppliers of PCR kits
Bangs Laboratories, our fine sponsors and suppliers of silica beads
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