Team:TU-Munich/Notebook/Materials

From 2013.igem.org

Revision as of 16:07, 23 August 2013 by FlorianA (Talk | contribs)

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        "TUM13_katrin-t.png",
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        "TUM13_rosario-t.png",
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        "TUM13_rosario-b-l.png",
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        "TUM13_rosario-t-r.png",
        "TUM13_fabian-front.png",        // Fabian
        "TUM13_fabian-t.png",
        "TUM13_fabian-t-l.png",
        "TUM13_fabian-l.png",
        "TUM13_fabian-b-l.png",
        "TUM13_fabian-b.png",
        "TUM13_fabian-b-r.png",
        "TUM13_fabian-r.png",
        "TUM13_fabian-t-r.png",
        "TUM13_andreas-front.png",        // Andreas
        "TUM13_andreas-t.png",
        "TUM13_andreas-t-l.png",
        "TUM13_andreas-l.png",
        "TUM13_andreas-b-l.png",
        "TUM13_andreas-b.png",
        "TUM13_andreas-b-r.png",
        "TUM13_andreas-r.png",
        "TUM13_andreas-t-r.png",
        "TUM13_louise-front.png",        // Louise
        "TUM13_louise-t.png",
        "TUM13_louise-t-l.png",
        "TUM13_louise-l.png",
        "TUM13_louise-b-l.png",
        "TUM13_louise-b.png",
        "TUM13_louise-b-r.png",
        "TUM13_louise-r.png",
        "TUM13_louise-t-r.png",
        "TUM13_johanna-front.png",        // Johanna
        "TUM13_johanna-t.png",
        "TUM13_johanna-t-l.png",
        "TUM13_johanna-l.png",
        "TUM13_johanna-b-l.png",
        "TUM13_johanna-b.png",
        "TUM13_johanna-b-r.png",
        "TUM13_johanna-r.png",
        "TUM13_johanna-t-r.png",
        "TUM13_meike-front.png",        // Meike
        "TUM13_meike-t.png",
        "TUM13_meike-t-l.png",
        "TUM13_meike-l.png",
        "TUM13_meike-b-l.png",
        "TUM13_meike-b.png",
        "TUM13_meike-b-r.png",
        "TUM13_meike-r.png",
        "TUM13_meike-t-r.png",
        "TUM13_volker-front.png",        // Volker
        "TUM13_volker-t.png",
        "TUM13_volker-t-l.png",
        "TUM13_volker-l.png",
        "TUM13_volker-b-l.png",
        "TUM13_volker-b.png",
        "TUM13_volker-b-r.png",
        "TUM13_volker-r.png",
        "TUM13_volker-t-r.png",
        "TUM13_polte-front.png",        // Polte
        "TUM13_polte-t.png",
        "TUM13_polte-t-l.png",
        "TUM13_polte-l.png",
        "TUM13_polte-b-l.png",
        "TUM13_polte-b.png",
        "TUM13_polte-b-r.png",
        "TUM13_polte-r.png",
        "TUM13_polte-t-r.png",
        "TUM13_leonie-front.png",        // Leonie
        "TUM13_leonie-t.png",
        "TUM13_leonie-t-l.png",
        "TUM13_leonie-l.png",
        "TUM13_leonie-b-l.png",
        "TUM13_leonie-b.png",
        "TUM13_leonie-b-r.png",
        "TUM13_leonie-r.png",
        "TUM13_leonie-t-r.png",
        "TUM13_philipp-front.png",        // Philipp
        "TUM13_philipp-t.png",
        "TUM13_philipp-t-l.png",
        "TUM13_philipp-l.png",
        "TUM13_philipp-b-l.png",
        "TUM13_philipp-b.png",
        "TUM13_philipp-b-r.png",
        "TUM13_philipp-r.png",
        "TUM13_philipp-t-r.png",
        "TUM13_jeff-front.png",        // Jeff
        "TUM13_jeff-t.png",
        "TUM13_jeff-t-l.png",
        "TUM13_jeff-l.png",
        "TUM13_jeff-b-l.png",
        "TUM13_jeff-b.png",
        "TUM13_jeff-b-r.png",
        "TUM13_jeff-r.png",
        "TUM13_jeff-t-r.png",
        "TUM13_chris-front.png",        // Chris
        "TUM13_chris-t.png",
        "TUM13_chris-t-l.png",
        "TUM13_chris-l.png",
        "TUM13_chris-b-l.png",
        "TUM13_chris-b.png",
        "TUM13_chris-b-r.png",
        "TUM13_chris-r.png",
        "TUM13_chris-t-r.png",
        "TUM13_flo-front.png",        // Flo
        "TUM13_flo-t.png",
        "TUM13_flo-t-l.png",
        "TUM13_flo-l.png",
        "TUM13_flo-b-l.png",
        "TUM13_flo-b.png",
        "TUM13_flo-b-r.png",
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Reagents

Chemicals

Reagent Supplier

Kits and Standards

Plastic Disposables

Oligonucleotides

Name Sequence
EreA_for 5’- GCGCGTCTAGATGGCCGGCACGTGGAGAACGACCAGA -3‘
EreA-rev 5‘- TTCGAAACCGGTTAGGGCAACTAGGCTGTCC -3‘
EreA_SDMI_for 5’- CGAATCAGGAAGAAAACTACAGTTAGTCGGAATCGAC -3‘
EreA_SDMI_rev 5'- GTCGATTCCGACTAACTGTAGTTTTCTTCCTGATTCG -3'
EreA_SDMII_for 5'- GACGCCTGTGGTACGGAAAATTCATGTCTGACATT - 3'
EreA_SDMII_rev 5'- AATGTCAGACATGAATTTTCCGTACCACAGGCGTC - 3'
EreA_SDMIII_for 5'- GTGTTCCAAGCGCGGGCAAGGACAGCC - 3'
EreA_SDMIII_rev 5'- GGCTGTCCTTGCCCGCGCTTGGAACAC - 3'
EreB_for 5'- GCGCGTCTAGATGGCCGGCAGGTTCGAAGAATGGGTC - 3'
EreB-rev 5'- TTCGAAACCGGTTTCATAAACGACCTCAGATACAG - 3'
EreB_SDMI_for 5'- GGTGAAAATTCTCATTTCATAAAAGAGTTCTTTTTGTTACGACATACGCTTTT - 3'
EreB_SDMI_rev 5'- AAAAGCGTATGTCGTAACAAAAAGAACTCTTTTATGAAATGAGAATTTTCACC - 3'
EreB_SDMII_for 5'- CTTATAGTGGGCATACAGCAGCCCTCTATCCGG - 3'
EreB_SDMII_rev 5'- CCGGATAGAGGGCTGCTGTATGCCCACTATAAG - 3'
EreB_SDMIII_for 5'- CGAGTTGATAACTTCCAACTACAGGAACCAAATGAAGGTTC - 3'
EreB_SDMIII_rev 5'- GAACCTTCATTTGGTTCCTGTAGTTGGAAGTTATCAACTCG - 3'
TEV_fw 5'- GCGCGTCTAGATGGCCGGCGGAGAAAGCTTGTTTAAGGGGC - 3'
TEV_rv 5'- ATATAACCGGTATTCATGAGTTGAGTCGCTTCC - 3'
TEV_t387c_fw 5'- ATGGTGTCAGACACTAGCTGCACATTCCCTTCATC - 3'
TEV_t387c_rv 5'- GATGAAGGGAATGTGCAGCTAGTGTCTGACACCAT - 3'
N_split_TEV_fw 5'- ATTCGCGGCCGCTTCTAG - 3'
N_split_TEV_rv 5'- TTCGAAACCGGTAGTTTGGAAGTTGGTTGTCAC - 3'
C_split_TEV_fw 5'- AAGCTTTCTAGATGGCCGGCAAGAGCATGTCTAGCATGGTG - 3'
C_split_TEV_rv 5'- CCGCTACTAGTATTAACCGG - 3'
PIF6_2-100_fw 5'- AAGCTTTCTAGATGGCCGGCATGTTCTTACCAACCGATTATTGTTGC - 3'
PIF6_2-100_rv 5'- TTCGAAACCGGTGTCAACATGTTTATTGCTTTC - 3'
MinMCS_fo 5'- CTAGACCTAGGAAGCTTCCTGCAGGACTAGTAAGCTTCTGCA - 3'
MinMCS_rev 5'- GAAGCTTACTAGTCCTGCAGGAAGCTTCCTAGGT - 3'
Bpu_for 5'- GCGCGTCTAGATGGCCGGCAACCTAGAAAAATTTGTTGACGAGCTG - 3'
Bpu_rev 5'- TTCGAAACCGGTGATGATATCCATCGGCCGCAT - 3'
Bpu_SDMI_for 5'- CACTTAAGCATTTTCTACGGGTCGATCACACCATTCA - 3'
Bpu_SDMI_rev 5'- TGAATGGTGTGATCGACCCGTAGAAAATGCTTAAGTG - 3'
Bpu_SDMII_fo 5'- GACACGATTAAATGTGTATGCGGGCTTAGCTGGATTTTATTTG - 3'
Bpu_SDMII_re 5'- CAAATAAAATCCAGCTAAGCCCGCATACACATTTAATCGTGTC - 3'
Bpu_SDMIII_fo 5'- CACCCTTAAAAATAAAGCTGGCTGCGGACAGGAAG - 3'
Bpu_SDMIII_re 5'- TTCCTGTCCGCAGCCAGCTTTATTTTTAAGGGTG - 3'
Bpu_SDMIV_fo 5'- CGTACGCTCACTCTTACGGGTACACAGGATAAATA - 3'
Bpu_SDMIV_re 5'- TATTTATCCTGTGTACCCGTAAGAGTGAGCGTACG - 3'
xylE_for 5'- AAGCTTTCTAGATGGCCGGCAACAAAGGTGTAATGCGACCG - 3'
xylE_rev 5'- TTCGAAACCGGTGGTCAGCACGGTCATGAATC - 3'
xylE_c312a_for 5'- GAACAGTTGTGGACGGCGCGTGCGC - 3'
xylE_c312a_rev 5'- GCGCACGCGCCGTCCACAACTGTTC - 3'
xylE_g483a_for 5'- CCTCATGTATGGCGACGAATTACCGGCGACCTA - 3'
xylE_g483a_rev 5'- TAGGTCGCCGGTAATTCGTCGCCATACATGAGG - 3'
xylE_a834g_for 5'- CAACTACCCGGACCACAAGCCGGTGACCT - 3'
xylE_a834_rev 5'- AGGTCACCGGCTTGTGGTCCGGGTAGTTG - 3'
p Act_for 5'- AAGCTTTCTAGAGTCGACGAGGTCATTCATATG - 3'
pAct_rev 5'- CTCGAGCTGCAGCGGCCGCTACTAGTAGCTTCTACCTACAAAAAAGCTC - 3'
t35S_for 5'- AAGCTTTCTAGAGCTAGAGTCCGCAAAAATCACC - 3'
t35S_rev 5'- CTCGAGCTGCAGCGGCCGCTACTAGTAGGTCACTGGATTTTGGTTTTAGG - 3'
Npt_for 5'- AAGCTTGAATTCGCGGCCGCTTCTAGAGATCGGATCCTGTCAAACAC - 3'
Npt_rev 5'- CGGCCGCTACTAGTAATCAAGCTTGACAGGAGGC - 3'
miniMCSII_fw 5'- CTAGACAATTGAAGCTTCCTGCAGGACTAGTAAGCTTCTGCA - 3'
miniMCSII_rv 5'- GAAGCTTACTAGTCCTGCAGGAAGCTTCAATTGT - 3'
SpyTag_fw 5'- CTAGATGGCCGGCGCTCATATTGTCATGGTTGATGCTTACAAGCCAACTAAGA - 3'
SpyTag_rv 5'- CCGGTCTTAGTTGGCTTGTAAGCATCAACCATGACAATATGAGCGCCGGCCAT - 3'
PIF3(2-100)_fw 5'- AAGCTTTCTAGATGGCCGGCCCTCTGTTTGAACTTTTCAGG - 3'
PIF3(2-100)_rv 5'- CCGCTACTAGTATTAACCGG - 3'
NucA_fw 5'- AAGCTTTCTAGATGGCCGGCGCAACTTCAACTAAAAAATTACATAAAGAACCTG - 3'
NucA_rv 5'- TTCGAAACCGGTTTGACCTGAATCAGCGTTGTC - 3'
AlcR_fw 5'- AAGCTTGAATTCGCGGCCGCTTCTAGATGGCAGATACGCGCCGAC - 3'
AlcR_rv 5'- TTCGAAACTAGTACTACAAAAAGCTGTCAACTTTCCCATTCAAACC - 3'
SQ_pAutoRex8_1 5'- CGGGGCATCACAGCGTCG - 3'
SQ_pAutoRex8_2 5'- TATCGGTAACCGAGTCCG - 3'
AlcR_QCI_fw 5'- GGCTAAATGTTCCTGCTGACATAGCCGCCGATG - 3'
AlcR_QCI_rv 5'- CATCGGCGGCTATGTCAGCAGGAACATTTAGCC - 3'
AlcR_QCII_fw 5'- GCACGGTATGGGTGCACGTTTGGACAAGCTACTCGAAAATG - 3'
AlcR_QCII_rv 5'- CATTTTCGAGTAGCTTGTCCAAACGTGCACCCATACCGTGC - 3'
AlcR_QCIII_fw 5'- CATCCAGTCTTGGTACGTCATTTTGGACGGTCACTGGCAT - 3'
AlcR_QCIII_rv 5'- ATGCCAGTGACCGTCCAAAATGACGTACCAAGACTGGATG - 3'
AlcR_QCIV_fw 5'- GCCGCAGCTGCAAGTACCCCGCTAG - 3'
AlcR_QCIV_rv 5'- ATTTTTGAGAAAAATATATATTCAGGCGAATTGCACAATGAACAATAATAAGATTAAAATAG - 3'
pActin_QC_fw 5'- ATTTTTGAGAAAAATATATATTCAGGCGAATTGCACAATGAACAATAATAAGATTAAAATAG - 3'
pActin_QC_rv 5'- CTATTTTAATCTTATTATTGTTCATTGTGCAATTCGCCTGAATATATATTTTTCTCAAAAAT - 3'
NPT_QCI_fw 5'- GTCCGGTGCCCTGAATGAATTGCAGGACGAG - 3'
NPT_QCI_rv 5'- CTCGTCCTGCAATTCATTCAGGGCACCGGAC - 3'
NPT_QCII_fw 5'- GCGGGACCCAAGCTTTAGATCTTGCTGCG - 3'
NPT_QCII_rv 5'- CGCAGCAAGATCTAAAGCTTGGGTCCCGC - 3'
IRES_polio_fw 5'- AAGCTTGAATTCGCGGCCGCTTCTAGAGTTAAAACAGCTCTGGGGTTG - 3'
IRES_polio_rv 5'- TTGCAACTGCAGCGGCCGCTACTAGTAAATCCAATTCGCTTTATGATAACAATC - 3'
BPU_SDMI_r_for 5'- CCACTTAAGCATTTTCTACCAGTCGATCACACCATTCACG - 3'
BPU_SDMI_r_rev 5'- CGTGAATGGTGTGATCGACTGGTAGAAAATGCTTAAGTGG - 3'
Strep-TEV_for 5'- CTAGATGGCCGGCAGCGCTTGGTCTCATCCTCAATTTGAAAAGGGTGAAAATCTTTATTTTCAAAGCGGAA - 3'
Strep-TEV_rev 5'- CCGGTTCCGCTTTGAAAATAAAGATTTTCACCCTTTTCAAATTGAGGATGAGACCAAGCGCTGCCGGCCAT - 3'
SQ_pluc-Actin_1 5'- CCGACTCAAATACAGATATGC - 3'
SQ_pluc-Actin_2 5'- CTCGGATGTAGATCTGCG - 3'
pActin_QC_ins_fw 5'- ACCCCCCCCTCTCCTCCCATCCCCCCAACCCTACCACCACCACC
ACCACCACCTCCACCTCCTCCCCCCTCGCTGCCGGACG - 3'
pActin_QC_ins_rv 5'- CGTCCGGCAGCGAGGGGGGAGGAGGTGGAGGTGGTGGTGGTGGT
GGTGGTAGGGTTGGGGGGATGGGAGGAGAGGGGGGGGT - 3'
pActin_Oryza_fw 5'- AAGCTTTCTAGAGTAGCTAGCATACTCGAGGTCATTCATATGCTTG - 3'
pActin_Oryza_rv 5'- TTCGAACTGCAGCGGCCGCTACTAGTACTTCTACCTACAAAAAAGCTCCG - 3'
GGGGSx5_TEV_fw 5'- CTAGATGGCCGGCGGTGGAGGCGGTTCAGGCGGAGGTGGCTCTGGCGGTGGCGGATCGGGAGG
CGGTGGCTCCGGAGGTGGAGGCTCAGGAGAGAATTTGTATTTTCAGTCAGGAA - 3'
GGGGSx5_TEV_rv 5'- CCGGTTCCTGACTGAAAATACAAATTCTCTCCTGAGCCTCCACCTCCGGAGCCACCGCCTCCC
GATCCGCCACCGCCAGAGCCACCTCCGCCTGAACCGCCTCCACCGCCGGCCAT - 3'
mMCSII_fw 5'- AATTCGCGGCCGCTTCTAGAGCAATTGAAGCTTCCTGCAGGTA - 3'
mMCSII_rv 5'- CTAGTACCTGCAGGAAGCTTCAATTGCTCTAGAAGCGGCCGCG - 3'
FluA_tripmut_fw 5'- AAGCTTTCTAGATGGCCGGCGACGTGTACCACGACGGTG - 3'
FluA_tripmut_rv 5'- TTCGAAACCGGTATTGTTGACCTTGCAGGCGG - 3'
pActin_QCI_fw 5'- CATAATTTTTGAGAAAAATATATATTCAGGCGAATTGTCACAATGAACAATAATAAGATTAAAATA - 3'
pActin_QCI_rv 5'- TATTTTAATCTTATTATTGTTCATTGTGACAATTCGCCTGAATATATATTTTTCTCAAAAATTATG - 3'
Alc R fw Seq. 5'- CAGAGCACAATTGTCCATAC - 3'
Alc R rv Seq. 5'- TCGCGGTCGATGCTCTCC - 3'
pActin_QCins_fw 5'- CATCGCCACTATATACATACCCCCCCCTCTCCTCCCATCCCCCCAACCCTACCACCACCACCAC
CACCACCTCCACCTCCTCCCCCCTCGCTGCCGGACGACGAGCTCCTC - 3'
pActin_QCins_rv 5'- GAGGAGCTCGTCGTCCGGCAGCGAGGGGGGAGGAGGTGGAGGTGGTGGTGGTGGTGGTGGTAGGG
TTGGGGGGATGGGAGGAGAGGGGGGGGTATGTATATAGTGGCGATG - 3'
PP1_for 5'- GCCGCTTCTAGATGGCCGGCGGATCCGCGGATTTAGATA - 3'
PP1_rev 5'- GTATTAACCGGTTTTCTTTGCTTGCTTTGTGATC - 3'
PhyB fw Seq 5'- GCGATTTCTCAGTTACAGGC - 3'
PhyB rv Seq. 5'- CTCAAAGCACGAGAAAGAAGC - 3'
pAct_ins_fw 5'- ACCACCACCACCTCCACCTCCTCCCCCCTCGCTGCCGGACGACGAGCTC - 3'
pAct_ins_rv 5'- GGTGGTGGTAGGGTTGGGGGGATGGGAGGAGAGGGGGGGGTATGTATATAGTGGCGATGG - 3'
PppAct_fw 5'- AAGCTTGAATTCGCGGCCGCTTCTAGATCGAGTCCTTGTGACTTTTGTGCAT - 3'
PppAct_rv 5'- TTCGAAACTAGTATATTTAATACGGACCTGCACAACAACCACCAAT - 3'
PppActin_QC1_fw 5'- TTATTGAGGATATCCATCTATCTTGATTTTGGACAATGTTTTACTGC - 3'
PppActin_QC1_rv 5'- GCAGTAAAACATTGTCCAAAATCAAGATAGATGGATATCCTCAATAA - 3'
PppActin_QC2_fw 5'- ACACTCTACATTCATGTCAAGAGTATAGGGACTTGGG - 3'
PppActin_QC2_rv 5'- CCCAAGTCCCTATACTCTTGACATGAATGTAGAGTGT - 3'
PppActin_fw_corr 5'- AAGCTTGAATTCGCGGCCGCTTCTAGAGCGAGTCCTTGTGACTTTTGTGCAT - 3'
SYFP2_fw 5'- AAGCTTTCTAGATGGCCGGCGTTAGCAAGGGCGAAGAAC - 3'
SYFP2_rv 5'- TTCGAAACCGGTTTTATACAGCTCATCCATACCCAG - 3'
eCFP_fw 5'- AAGCTTTCTAGATGGCCGGCGTGAGCAAGGGCGAGGAG - 3'
eCFP_rv 5'- TTCGAAACCGGTCTTGTACAGCTCGTCCATGCC - 3'
G-TEV-site-G_fw 5'- CTAGATGGCCGGCGGAGAGAATTTGTATTTTCAGTCAGGAA - 3'
G-TEV-site-G_fw 5'- CCGGTTCCTGACTGAAAATACAAATTCTCTCCGCCGGCCAT - 3'
PppActin_seq_fw 5'- CATGTCCCAGGGCCCTAG - 3'

Instruments

Electrophoresis

Photometers

Nanodrop Spectrohpotometer

Moss-cultivation

References:

http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984

  1. http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984 Edens, L., Bom, I., Ledeboer, A. M., Maat, J., Toonen, M. Y., Visser, C., and Verrips, C. T. (1984). Synthesis and processing of the plant protein thaumatin in yeast. Cell, 37(2):629–33.


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